Recombinant Human Factor VIII

Factor-VIII Human Recombinant
Factor-VIII Human Recombinant
Catalog No. PRO-318

Synonyms:
Coagulation factor VIII, Procoagulant component, Antihemophilic factor, AHF, F8, F8C, F8B, HEMA, FVIII, DXS1253E, F8 protein.

Introduction:
Coagulation factor VIII participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.

Description:
Antihemophilic Facor Human Recombinant produced in CHO is a glycosylated polypeptide chain having a total amino acids of 1438 (170kd) and consisting of two dimer chains 80 kD and 90 kD.
The Factor-VIII is purified by proprietary chromatographic techniques.

Source:
CHO cells (Chinese Hamster Ovarian Cells)

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein was lyophilized from a solution containing 50mM histidine, 0.3M NaCl, 5mM CaCl2, 0.02% Tween 80, 20mM sucrose, pH- 6.8.

Solubility:
It is recommended to reconstitute the lyophilized Factor-VIII in 1ml sterile 18MΩ-cm H2O, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Factor-VIII although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Factor-VIII should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity:
Greater than 97.0% as determined by SDS-PAGE.

Biological Activity:
The specific activity was found to be 6839IU/mg.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

» Factor IX Human
» Factor VIIa Human
» Factor VIII RA Human
» Factor-IX Antibody
» Factor-VIII Human