重组人钙结合蛋白D-29K(Recombinant Human CaBP29K)

CaBP29K Human
Calretinin Human Recombinant
Catalog No. PRO-401

Synonyms:
Calretinin, CR, CALB2, CAB29, CAL2, CaBP29K, 29 kDa calbindin.

Description:
Calretinin/CR/CLB2/CALB2/Calbindin D29K protein (mouse/rat/human 271aa, chromosome 16q22.1, mol wt ~29 kDa) also belongs to the Calbindin family. It is most closely related to CABP28K (~55% identity). It may be alternatively spliced to a C-terminally truncated fragment, Calretinin-22K in some tumor cell lines. Calretinin is highly expressed in the cerebellum, olfactory bulb, and in auditory neurons. Calretinin gene inactivation in mice eliminated long-term potentiation induction in the gentate gyrus and impaired motor coordination.
Recombinant Human Calbindin 29kDa is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris pH-7.4, 50mM sodium chloride and 1mM b-mercaptoethanol.

Solubility:
It is recommended to reconstitute the lyophilized CABP29K in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized CABP29K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP29K should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 90% as determined by SDS-PAGE.

Applications:
This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For adsorption we suggest the following procedure:

A- Dilute 1 µl of the antiserum against calretinin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).
B- Add 1µg of recombinant protein to 1ml of the diluted antibody solution and mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections and incubate for 3 days.
E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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