重组巴曲酶(Recombinant Batroxobin)

Batroxobin
Batroxobin Recombinant
Catalog No. PRO-607

Synonyms:
Thrombin-like enzyme batroxobin, EC 3.4.21.74, BX, Bothrops atrox serine proteinase, Venombin-A, Defibrase, Reptilase, Batroxobin.

Introduction:
Batroxobin is a serin protease that reduces fibronogen levels and is originally extracted from snake venom of Bothrops Atrox. Batroxobin is used in defibrinogenation and thrombolysis and also has an effect on c-fos gene and growth factor.
Batroxobin can efficiently restrain proliferation of VSMCs, by blocking the release and uptake of Ca2+, thus influencing [Ca2+]i.
Batroxobin is a single chain glycopeptide with a molecular mass of 43kDa on SDS-PAGE gel and its pI-6.6. Batroxobin converts fibrinogen to fibrin through the restricted release of fibrinopeptide-A from fibrinogen to promote blood to clot. Unlike thrombin, it is not affected by heparin and hirudin.

Description:
The Batroxobin Recombinant Protein, produced in yeast, is a single, glycosilated polypeptide chain containing 213 amino acids and having a 28-33 kDa.

Source:
Pichia Pastoris

Physical Appearance:
Sterile Filtered liquid formulation.

Formulation:
The Batroxobin protein solution contains 20mM sodium acetate buffer, pH 7.4.

Stability:
Batroxobin although stable at 15°C for 2 weeks, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity:
Batroxobin’s biological activity was found to be of no less than 500KU/mg. (Klobusitzky Unit).

Amino Acid Sequence:
VIGGDECDIN EHPFLAFMYY SPRYFCGMTL INQEWVLTAA HCNRRFMRIH
LGKHAGSVAN YDEVVRYPKE KFICPNKKKN VITDKDIMLI RLDRPVKNSE
HIAPLSLPSN PPSVGSVCRI MGWGAITTSE DTYPDVPHCA NINLFNNTVC
REAYNGLPAK TLCAGVLQGG IDTCGGDSGG PLICNGQFQG ILSWGSDPCA
EPRKPAFYTK VFDYLPWIQS IIAGNKTATC P.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.