重组人硫嘌呤甲基转移酶(Human TPMT)

TPMT Human
Thiopurine S-methyltransferase Human Recombinant
Catalog No. PKA-255

Synonyms:
TPMT, Thiopurine S-methyltransferase, EC 2.1.1.67, Thiopurine methyltransferase.

Introduction:
TPMT, thiopurine S-methyltransferase, is a cytosolic enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity.

Description:
TPMT Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 245 amino acids (1-245) and having a molecular mass of 28 kDa.
Thiopurine S-methyltransferase is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile filtered colorless solution.

Formulation:
1 mg/ml solution containing 20mM Tris 8.0, 0.2mM PMSF and 2mM EDTA.

Amino acid sequence:
MDGTRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGKTAF HQEQGHQLLK KHLDTFLKGKSGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIQEFF TEQNLSYSEE PITEIPGTKVFKSSSGNISL YCCSIFDLPR TNIGKFDMIW DRGALVAINP GDRKCYADTM FSLLGKKFQY LLCVLSYDPT KHPGPPFYVP HAEIERLFGK ICNIRRLEKV DAFEERHKSW GIDCLFEKLYLLTEK.

Stability:
Thiopurine S-methyltransferase Human Recombinant although stable at 4°C for 1 week, should be stored desiccated below -18°C.
Please prevent freeze thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.