重组人转化生长因子-β受体II(Human TGFBR2)

TGFBR2 Human
Transforming Growth Factor Beta Receptor II Human Recombinant
Catalog No. PKA-261

Synonyms:
AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII, TGFBR-2, TGF-beta receptor type-2, Transforming growth factor-beta receptor type II, TGF-beta receptor type II, TGF-beta type II receptor, TbetaR-II, TGFBR2.

Introduction:
TGFBR2 is part of the Ser/Thr protein kinase family and the TGFB receptor subfamily. TGFBR2 is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 gene have been associated with Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. TGFBR2 expression is increased in oral squamous cell carcinoma cells. TGFBR2 attenuates the biological activities of TGF-beta in colorectal cancer. TGFBR2 expression is decreased by IL-1beta while inducing Sp3 via NFkappaB. TGFB2 and TGFBR2 are involved in the antiestrogenic activity of tamoxifen metabolites in breast cancer.

Description:
TGFBR2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain encoding 133 amino acids (4-136 a.a.), the extracellular domain of TGFBR2, having a molecular mass of 15.1 kDa.
TGFBR2 is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
Lyophilized from a 0.2 μm filtered solution in buffer containing 150mM NaCl & 50mM sodium phosphate buffer pH-7.5.

Solubility:
It is recommended to reconstitute the lyophilized TGFBR2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized TGFBR2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFBR2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 97.0% as determined by SDS-PAGE.

Activity:
Measured by its ability to inhibit TGF-β1 activity on a mouse T cell line, HT-2 (Tsang, M. et al., 1995, Cytokine 7:389). The ED50 for this effect is typically 1 - 3 μg/mL in the presence of 1 ng/ml of rhTGF-β1. In ELISA assay concentrations 0.2-0.4 µg/ml of TβRII can be used for TGFβ measurements.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.