Recombinant Human STAT1

STAT1 Human
STAT1 Human Recombinant
Catalog No. PKA-315

Synonyms:
Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1, ISGF-3, STAT91, DKFZp686B04100.

Introduction:
STAT1 is a member of the Signal Transducers and Activators of Transcriptionfamily of transcription factors. STAT1 is involved in upregulating genes due to a signal by either type Ior type IIinterferons. In response to IFN-γstimulation, STAT1 forms homodimers or heterodimers with STAT3that bind to the GAS (Interferon-Gamma Activated Sequence) promoter element; in response to either IFN-α or IFN-βstimulation, STAT1 forms a heterodimer with STAT2that can bind the ISRE (Interferon Stimulated Response Element) promoter element. In either case, binding of the promoter element leads to an increased expression of ISG (Interferon Stimulated Genes).

Description:
STAT1 Human Recombinant produced in E.Coli is a non-glycosylated polypeptide chain having a molecular mass of 90 kDa. This protein is the full-length form of the protein with an amino-terminal His-tag.
The STAT1 is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
Lyophilized protein with no additives.

Stability:
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Unit Definition:
Recombinant human STAT1 is phosphorylatable in vitro, using either immunoprecipitated JAK1 or JAK2, or whole cell extracts derived from interferon-γ-stimulated HEK 293 cells. This phosphorylation can be monitored by Western blot analysis using phosphorylation site specific antibody directed to STAT [pY701], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.

Purity:
Greater than 95% as determined by SDS-PAGE.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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