PKC-g Human
Protein Kinase C gamma Human Recombinant
Catalog No. PKA-320
Synonyms:
Protein kinase C gamma type, EC 2.7.11.13, PKC-gamma, PRKCG, PKCC, PKCG, SCA14, MGC57564.
Introduction:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).
Description:
PKC-g Human Recombinant produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-676 and having a molecular mass of 80 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag.
PKC-g is purified by proprietary chromatographic techniques.
Source:
Sf9 insect cells.
Physical Appearance:
Sterile Filtered clear solution.
Formulation:
PKC-g is supplied at a of 0.1mg/ml in 10mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.
Unit Definition:
6000-8000 units/mg. One unit of PKC-g activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30°C. Recombinant human active PKC-g also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3b. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.
Storage Conditions:
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity:
Greater than 90% as determined by SDS-PAGE.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
» PKC-a Human
» PKC-b I Human
» PKC-b II Human
» PKC-d Human
» PKC-t Human
» PRKCE Human
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企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
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