重组人N-氨基末端激酶2 α1(Human JNK2a-1)

JNK2a-1 Human
JUN N-terminal Kinase 2 alpha 1 Human Recombinant
Catalog No. PKA-220

Synonyms:
Mitogen-activated protein kinase 9, EC 2.7.11.24, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, JNK-55, JNK2, JNK2A, JNK2B, PRKM9, JNK2BETA, p54aSAPK, JNK2ALPHA, MAPK9, JUN N-terminal Kinase 2 alpha 1, JNK2a-1.

Introduction:
JUN N-terminal Kinase 2is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Description:
JNK2 is a soluble 48 kDa protein that is activated in response to cellular stress, radiation and growth factors. JNK2 binds to the amino terminal activation domains of c-jun or ATF2 regulating AP-1 transcriptional activity.
This protein is the full-length form of the protein with a C-terminal His-tag (His-tag on COOH terminus).

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
25mM HEPES at pH 8.0, 150mM NaCl, 2mM DTT and 10% glycerol.

Unit Definition:
Activity is approximately 200 units/mg. A certificate of analysis is provided, stating the enzymatic activity for
each production lot. 1.0 unit of activity is defined as the amount of enzyme required to catalyze the transfer of
nmole of phosphate from ATP to to a protein substrate per minute at 30ºC.

Storage Conditions:
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity:
Greater than 85% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.

Amino Acid Sequence:
MSDSKCDSQF YSVQVADSTF TVLKRYQQLK PIGSGAQGIV CAAFDTVLGI NVAVKKLSRP FQNQTHAKRAYRELVLLKCV NHKNIISLLN VFTPQKTLEE FQDVYLVMEL MDANLCQVIH MELDHERMSY LLYQMLCGIKHLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTACTNF MMTPYVVTRY YRAPEVILGM GYKENVDIWSVGCIMGELVK GCVIFQGTDH IDQWNKVIEQ LGTPSAEFMK KLQPTVRNYV ENRPKYPGIK FEELFPDWIFPSESERDKIK TSQARDLLSK MLVIDPDKRI SVDEALRHPY ITVWYDPAEA EAPPPQIYDA QLEEREHAIEEWKELIYKEV MDWEERSKNG VVKDQPSDAA VSSNATPSQS SSINDISSMS TEQTLASDTD SSLDASTGPLEGCRGGGSHH HHHH

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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