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当前位置: 上海希美化学 > 常用生化试剂 > 重组尿素酶(Urease)

重组尿素酶(Urease)

供货周期: 一周
品牌: Prospect Biosystems
规格: 1mg/5mg/50mg
货号: ENZ-277
CAS号:
报价: 面议
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产品介绍

Urease
Urease Recombinant
Catalog No. ENZ-277

Description:
Urease (EC3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows:
(NH2)2CO+ H2O → CO2+ 2NH3
In 1926 James Sumner showed that urease is a protein. Urease is found in bacteria, yeast and several higher plants.
Characteristics: Active sitemetal: nickel(II); Molecular weight: 480 kDa or 545 kDa for Jack Bean Urease (calculated mass from the amino acid sequence); Optimum pH: 7.4; Optimum Temperature: 60 degrees Celsius; Enzymatic specificity: urea and hydroxyurea; Inhibitors: heavy metals.
The multi-subunit enzyme usually has a 3:3 (alpha:beta) stoichiometry with a 2-fold symmetric structure (note that the image above gives the structure of the asymmetric unit, one third of the true biological assembly). An exceptional urease is found in Helicobacter pylori, which combines four of the regular six subunit enzymes in an overall tetrahedral assembly of 24 subunits (α12β12). This supra-molecular assembly is thought to confer additional stability for the enzyme in this organism, which functions to produce ammonia in order to neutralise gastric acid. The presence of urease is used in the diagnosis of Helicobacter species.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Lyophilized Powder.

Formulation:
Each mg of protein contains 345µg Potassium Phosphate and 25µg EDTA Na2.

Solubility:
It is recommended to reconstitute the lyophilized Urease in sterile 18MΩ-cm H2O.

Stability:
Urease although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity:
The specific activity was found to be 150 IU/mg.

Unit Definition:
One Unit oxidizes one micromole of NADH per minute at 25°C, at pH 7.6.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

工商信息

企业名称

上海希美化学有限公司

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企业类型

信用代码

310228001203103

成立日期

2009-05-31

注册资本

10

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重组尿素酶(Urease)由上海希美化学有限公司为您提供,货号ENZ-277,规格:1mg/5mg/50mg,CAS号:,如您想了解更多关于重组尿素酶(Urease)价格、重组尿素酶(Urease)结构式、批发、用途等信息,欢迎咨询。除供应重组尿素酶(Urease)外,还可为您提供人丙酮酸激酶M2型同工酶(M2-PK)ELISA Kit、牛磷酸烯醇式丙酮酸羧激酶(PCK)ELISA Kit 、小鼠丙酮酸脱氢酶E1(PDH E1) ELISA Kit等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,上海希美化学客户服务电话,售前、售后均可联系。
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