Urease
Urease Recombinant
Catalog No. ENZ-277
Description:
Urease (EC3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows:
(NH2)2CO+ H2O → CO2+ 2NH3
In 1926 James Sumner showed that urease is a protein. Urease is found in bacteria, yeast and several higher plants.
Characteristics: Active sitemetal: nickel(II); Molecular weight: 480 kDa or 545 kDa for Jack Bean Urease (calculated mass from the amino acid sequence); Optimum pH: 7.4; Optimum Temperature: 60 degrees Celsius; Enzymatic specificity: urea and hydroxyurea; Inhibitors: heavy metals.
The multi-subunit enzyme usually has a 3:3 (alpha:beta) stoichiometry with a 2-fold symmetric structure (note that the image above gives the structure of the asymmetric unit, one third of the true biological assembly). An exceptional urease is found in Helicobacter pylori, which combines four of the regular six subunit enzymes in an overall tetrahedral assembly of 24 subunits (α12β12). This supra-molecular assembly is thought to confer additional stability for the enzyme in this organism, which functions to produce ammonia in order to neutralise gastric acid. The presence of urease is used in the diagnosis of Helicobacter species.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Lyophilized Powder.
Formulation:
Each mg of protein contains 345µg Potassium Phosphate and 25µg EDTA Na2.
Solubility:
It is recommended to reconstitute the lyophilized Urease in sterile 18MΩ-cm H2O.
Stability:
Urease although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity:
The specific activity was found to be 150 IU/mg.
Unit Definition:
One Unit oxidizes one micromole of NADH per minute at 25°C, at pH 7.6.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
更多
更多
企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
上海希美化学有限公司
公司地址
上海市松江区陆家圈路168弄113号
客服电话