UNG
Uracil DNA Glycosilase
Catalog No. ENZ-352
Synonyms:
Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG.
Description:
E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).
Source:
Escherichia Coli strain that carries the UNG gene from E.coli
Physical Appearance:
Sterile Filtered colorless solution.
Formulation:
10mM Tris-Hcl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.
Stability:
Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.
Applications:
Glycosilase mediated single nucleotide polymorphism detection (GMPD).
Site-directed mutagenesis.
As a probe for protein-DNA interaction sudies.
Rapid and efficient cloning of PCR products.
Elimination carry-over contamination in PCR.
Specific Activity:
The Specific Activity was found to be 5U/μl.
Unit Definition:
1 Unit is defined as the amount of UDG enzyme that catalyzes release of 60pmol of Uracil per minute from double-stranded, uracil-containing DNA in a total reaction volume of 50µl in 30 minutes at 37°C in 1X Uracil DNA Glycosilase Reaction Buffer with 1 Unit of Uracil DNA Glycosylase and 0.2µg [3H]-Uracil DNA (104- 105 cpm/µg)
10X UNG Reaction Buffer:
200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA.
Reaction Conditions:
1X UNG Reaction Buffer, incubate at 37°C.
UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
更多
更多
企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
上海希美化学有限公司
公司地址
上海市松江区陆家圈路168弄113号
客服电话