T重组人DNA拓扑异构酶(Human OP1)

TOP1 Human
DNA Topoisomerase-I Human Recombinant
Catalog No. ENZ-306

Synonyms:
DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70.

Introduction:
DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis.
In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100 kDa molecular weight. Most of this precursor is then proteolytically processed to a species with 70 kDa molecular weight from which the Scl-70 antigen has derived its name.

Description:
DNA Topoisomerase-I Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 Dalton.
The TOP1is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Source:
Sf9 insect cells.

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
TOP1 is supplied in 16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol.

Immunological functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

Coating concentration:
0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Storage Conditions:
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity:
Greater than 80% as determined by SDS-PAGE.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.