PL 7 Human
Threonyl-tRNA Synthetase Human Recombinant
Catalog No. ENZ-304
Synonyms:
Threonyl-tRNA synthetase cytoplasmic, EC 6.1.1.3, Threonine-tRNA ligase, ThrRS, MGC9344, PL-7, TARS.
Introduction:
Threonyl-tRNA Synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 83 kDa protein is an autoantigen recognized by PL-7 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that PL-7 antibodies (similar to Jo-1 antibodies) indicate an increased risk for lung involvement, but this needs to be confirmed for a larger number of cases.
Description:
Threonyl-tRNA Synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 83,312 Dalton.
PL-7 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Source:
Sf9 insect cells.
Physical Appearance:
Sterile Filtered clear solution.
Formulation:
PL-7 is supplied in 20mM HEPES buffer pH-8, 200mM sodium chloride, and 20% glycerol.
Immunological functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).
Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.
Coating concentration:
0.3-0.8 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Storage Conditions:
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity:
Greater than 90% as determined by SDS-PAGE.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
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