重组人亚胺甲基转移酶环托氨酶(Human FTCD)

FTCD Human
Formiminotransferase Cyclodeaminase Human Recombinant
Catalog No. ENZ-302

Synonyms:
Formiminotransferase-cyclodeaminase, FTCD, LCHC1, LC-1.

Introduction:
Formiminotransferase cyclodeaminase is an enzyme which catalyzes the conversion of formiminoglutamateand tetrahydrofolateinto formiminotetrahydrofolateand glutamate.
Formiminotransferase cyclodeaminase, a bifunctional enzyme of tetrahydrofolate synthesis, is the target antigen of anti-LC1 (liver cytosol antigen type 1) autoantibodies. Presence of LC1 autoantibodies is a marker for type 2 autoimmune hepatitis (for which anti-LKM 1/cytochrome P450 2D6 autoantibodies are a further marker).
Serum LC1 autoantibody concentrations appear to fluctuate in parallel with aminotransferase levels, a particularly intriguing observation that suggests a possible role of LC1 autoreactivity in the pathogenic mechanism leading to hepatocyte injury.

Description:
Formiminotransferase Cyclodeaminase Human Recombinant (also called liver cytosol type 1) produced in SF9, is a glycosylated, polypeptide chain having a molecular mass of 59,749 Dalton.
The FTCD is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Source:
Sf9 insect cells.

Formulation:
FTCD is supplied at a concentration of 0.5mg/ml in 16mM HEPES buffer pH-7.6, 240mM sodium chloride, and 20% glycerol.

Immunological functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).

Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody.and autoimmune hepatitis (AIH II) patient
sera.

Coating concentration:
0.35-0.7 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Storage Conditions:
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°Cfor longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity:
Greater than 95% as determined by SDS-PAGE.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.