辣根过氧化物酶(Horseradish Peroxidase/HRP)

HRP
Horseradish Peroxidase
Catalog No. ENZ-321

Synonyms:
Horseradish Peroxidase, HRP, EC 1.11.1.7.

Introduction:
The enzyme horseradish peroxidase, found in horseradish, is used extensively in molecular biologyand in antibody amplification and detection, among other things. For example, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase (HRP) has become a major tool. In its brief history, this method has probably been used by more neurobiologists than have used the Golgi stainsince its discovery in 1870." Horseradish peroxidase is also highly used in techniques such as Western blottingand ELISAs.
HRP is widely used as an enzymatic label in immunoassays. Usually, the enzyme is coupled to antibodies, lectins or haptens. Coupling to antibodies etc. may be performed through the carbohydrate side chains of the HRP.

Description:
HRP consists of the basic isoenzyme having a molecular weight of 44 kDa.
The Horseradish Peroxidase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.

Source:
Root extracts of horseradish

Physical Appearance:
Sterile Filtered red-brown lyophilized powder.

Solubility:
It is recommended to reconstitute the lyophilized HRP in sterile 18MΩ-cm H2O not less than 100 µg/ml or more than 10 mg/ml solutions.

Stability:
Lyophilized HRP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HRP should be stored at 4°C between 2-7 days and for future use below
-18°C.
Please prevent freeze-thaw cycles.

Activity:
Greater than 250 U/mg (25°C, guaiacol as the hydrogen donor, pH-7 and H2O2 as substrates).

Purity:
(A403/A278) = RZ: 3.5.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.