重组人胱天蛋白酶-3(Caspase-3/CASP3)

CASP3 Human, Active
Caspase-3 Human Recombinant, Active
Catalog No. ENZ-369

Synonyms:
Caspase 3, CASP-3, Apopain, CPP32, Yama protein, CPP-32, SREBP cleavage activity 1, SCA-1, SPP32B, EC 3.4.22.56.

Introduction:
CASP3 is part of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspase-3 enzyme results in the execution-phase of cell apoptosis/programmed cell death. Caspase 3 has been called the "henchman that goes around and executes the cell." Caspase3 exists as an inactive proenzyme which undergoes proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. CASP3 cleaves and activates caspases 6, 7 and 9, and be processed by caspases 8, 9 and 10. Caspase-3 is involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's & Huntington disease.
High levels of Caspase-3 are found in lymphocytes, indicating that it is an important mediator of apoptosis in the immune system.
CSN-mediated deneddylation is regulated by active CASP3.
CASP-3 cleavage of RAD51 results in a functional decrease in RAD51 strand exchange activity and inhibition of caspase 3 activity increases RAD51 protein levels.
Mice overexpressing human caspase 3 have increased susceptibility to degenerative insults. Single nucleotide polymorphisms in Caspase-3 gene is associated with lung cancer.
Caspase3 is activated by elaidic acid and palmitic acid.
CASP3 activity is enhanced by safrole oxidase in lung cancer cells.
Expression of CASP3 in peripheral blood mononuclear cells is significantly increased in SLE patients.

Description:
CASP3 Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain cotaning 258 amino acids and having a molecular mass of 29.7 kDa.
The Caspase-3 is fused to His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
The Caspase3 solution contains 50mM Hepes, pH-7.4 + 100mM NaCl, 0.1% Chaps, 10mM DTT & 10% glycerol.

Stability:
CASP3 should be stored desiccated below (-18°C) - (-80°C).
Please prevent freeze-thaw cycles.

Purity:
Greater than 90.0% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Cleavage conditions:
10mM Hepes, 7.4, 10mM DTT.

Activity:
At a concentration of 0.5 mg/ml one unit will cleave 1.0 umoles of substrate AC-DEVD-P Nitroanilide per minute at pH 7.4 at 25 degrees.

Protein Uses:
Caspase-3 should be preincubated with 10mM DTT at room temperature for one hour to fully reduce the enzyme, before cleavage begins. If product tolerates reduced conditions, assay can be carried out in 20mM Hepes, pH-7.4 + 150mM NaCl & 10mM DTT.

Amino Acid Sequence:
SGISLDNSYK MDYPEMGLCI IINNKNFHKS TGMTSRSGTD VDAANLRETF RNLKYEVRNK NDLTREEIVE LMRDVSKEDH SKRSSFVCVL LSHGEEGIIF GTNGPVDLKK ITNFFRGDRC RSLTGKPKLF IIQACRGTEL DCGIETDSGV DDDMACHKIP VEADFLYAYS TAPGYYSWRN SKDGSWFIQS LCAMLKQYAD KLEFMHILTR VNRKVATEFE SFSFDATFHA KKQIPCIVSM LTKELYFYHH HHHHHHHH

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.