Recombinant Human CD4 (125-202)

CD4 Human (125-202)
CD-4 (125-202 a.a.) Human Recombinant
Catalog No. CYT-315

Synonyms:
gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4.

Introduction:
CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with ~60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E- cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.

Description:
CD-4 Human Recombinant is fused with a 4kDa His Tag and encoding amino acids 125-202, having a total molecular weight of 19 kDa.

Source:
Escherichia Coli.

Physical Appearance:
Sterile lyophilized powder.

Formulation:
Each mg contains 1X PBS.

Solubility:
It is recommended to reconstitute the lyophilized CD-4 (125-202) in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Store vial at -20oC to -80oC. When stored at the recommended temperature, this protein is stable for 12 months.
Please avoid freeze-thaw cycles.

Purity:
Greater than 90% as determined by SDS-PAGE.

Applications:
• Protein Assay
• Antibody Production
• Western Blotting

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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