重组人肌肉生长抑制素Propetide(Human Myostatin Propetide)

Myostatin Propeptide Human
Myostatin Propeptide Human Recombinant
Catalog No. CYT-448

Synonyms:
GDF-8, MSTN, Growth Differentiation Factor 8, MSTN Muscle Hypertrophy.

Introduction:
Myostatin (GDF-8), a member of the TGFbeta superfamily, is a potent and specific negative regulator of skeletal muscle mass. In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide. The myostatin propeptide is negative regulator of myostatin in vivo.

Description:
28 kDa protein containing 243 amino acid residues of the human Myostatin Propeptide and 5 additional amino acid residues (underlined).
MGNENSEQKE NVEKEGLCNA CTWRQNTKSS RIEAIKIQIL SKLRLETAPN ISKDVIRQLL PKAPPLRELI DQYDVQRDDS SDGSLEDDDY HATTETIITM PTESDFLMQV DGKPKCCFFK FSSKIQYNKV VKAQLWIYLR PVETPTTVFV QILRLIKPMK DGTRYTGIRS LKLDMNPGTG IWQSIDVKTV LQNWLKQPES NLGIEIKALD ENGHDLAVTF PGPGEDGLNP FLEVKVTDTP KRSRRKLN

Specificity:
The amino acid sequence of the recombinant human Myostatin Propeptide is100% homologous to the amino acid sequence of the human Myostatin Propeptide without signal sequence.

Purification Method:
Two-step procedure using size exclusion chromatography before and after refolding.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.

Formulation:
Lyophilized from 10mM Acetic Acid.

Solubility:
It is recommended to reconstitute the lyophilized Myostatin Propeptide in sterile 20mM HCl at 0.1 mg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
The lyophilized protein remains stable until the expiry date when stored at -20°C.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Applications:
Western blotting, ELISA.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.

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