Recombinant Mouse FGF-2(重组小鼠成纤维细胞生长因子-2)

Prospec (蛋白质专家) 是一家领先的生物科技公司，有16年生产细菌重组蛋白方面的专业经验。Prospec还生产细胞因子，生长因子，趋化因子，激素，酶，病毒抗原及其它的重组蛋白。
Prospec的蛋白质提纯技术申请了专利，所以Prospec以生产成本低，生产效率高，产品纯度高，有竞争力的价格，使Prospec产品在市场上立于不败之地（占有很大优势）。
Prospec为满足顾客的需要，还根据顾客需求进行重组蛋白生产服务。
Prospec为全球的学术机构，政府研究部门，生物科技和药物公司以及医院和参照实

FGF 2 Mouse
Fibroblast Growth Factor-Basic Mouse Recombinant
Catalog No. CYT-386 
Synonyms:
HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b.

Introduction:
FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors.

Description:
Fibroblast Growth Factor-basic Mouse Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 154 amino acids and having a molecular mass of 17153 Dalton.
The FGF-2 is purified by proprietary chromatographic techniques.


Source:
Escherichia Coli.




Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
FGF-b was lyophilized from 1 mg/ml solution after extensive dialysis against 20mM phosphate buffer, pH 7.2, 50mM Na2SO4, 0.2mM DTT 0.2mM EDTA.


Solubility:
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor ß in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-basic should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.


Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Ser-Gly

Biological Activity:
The ED50 range as determined by the dose-dependant proliferation of BALB/3T3 cells was found to be less than 0.5 ng/ml, corresponding to a specific activity of 2.0 x 106 IU/mg.

Protein content:
Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.885 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of FGF2 as a Reference Standard.


Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.