Prospec (蛋白质专家) 是一家领先的生物科技公司,有16年生产细菌重组蛋白方面的专业经验。Prospec还生产细胞因子,生长因子,趋化因子,激素,酶,病毒抗原及其它的重组蛋白。
Prospec的蛋白质提纯技术申请了专利,所以Prospec以生产成本低,生产效率高,产品纯度高,有竞争力的价格,使Prospec产品在市场上立于不败之地(占有很大优势)。
Prospec为满足顾客的需要,还根据顾客需求进行重组蛋白生产服务。
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ANG 2 Human
Angiopoietin-2 Human Recombinant
Catalog No. CYT-414
Synonyms:
ANGPT2, Tie2-ligand, Ang-2, AGPT2, angiopoietin-2B, angiopoietin-2a, Angiopoietin-2.
Introduction:
The Angiopoietins are a family of growth factors which bind to the endothelial receptor tyrosine kinase Tie2. Angiopoietin-1, 4 activate the Tie2 receptor, whereas Angiopoietin-2, 3 inhibit Angiopoietin-1-induced Tie2 phosphorylation. Ang-1 Human is a secreted growth factor which enhances endothelial cell survival and capillary morphogenesis, also it limits capillary permeability. Ang-2 is a natural inhibitor of Ang-1 because it binds the same receptor but fails activate it. When ambient levels of VEGF are high Angiopoietin-2 destabilizes capillary integrity, facilitating sprouting but when VEGF levels are low it causes vessel regression. Although Tie-1 and Tie-2 homologues but Tie-1’s ligands are unknown. The mammalian metanephros (precursor of the adult kidney) is the local where Angiopoietin and Tie genes are expressed. These genes may play a role in endothelial precursor growth. When the metanephros first forms Tie-1-expressing cells can be detected. Ang-1 (podocyte-derived) and Ang-2 (mesangial-cell-derived) may affect growth of nascent capillaries during glomerular maturation. The descending limbs of loops of Henle in the mature vasa rectae (after birth) express Angiopoietin-2 which affects the growth of this medullary microcirculation. As gathered from recent data, angiopoietins are implicated in unregulated vessel growth in Wilms' kidney tumors and in vascular remodeling after nephrotoxicity. During vascular development VEGF-A and Angiopoietins not only have different roles, but also complementary and coordinated roles.
Description:
Angiopoitin-2 (Ang-2) Human, a 66 kDa protein consisting of 476 amino acid residues (N21-F496), is fused to a N-terminal myc-tag and produced in insect cells.
Source:
Insect Cells
Physical Appearance:
Sterile Filtered white lyophilized freeze dry powder.
Formulation:
Ang-2 Human is formulated from a solution containing TBS & 0.05% CHAPS.
Solubility:
It is recommended to reconstitute the lyophilized Angiopoitin-2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Angiopoietin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Ang-2 Human Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity:
The purity of Ang-2 Human is greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity:
The biological activity of Angiopoietin-2 was determined by the induction of endothelial cell sprouting as described in Korff et al., 2001.
Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of ANG-2 Human as a Reference Standard.
Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
上海希美化学有限公司
公司地址
上海市松江区陆家圈路168弄113号
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