人alpha突触核蛋白（淀粉样前体非A4成分蛋白）低聚物(SNCA oligomer)ELISA Kit

 产品名称： 人alpha突触核蛋白（淀粉样前体非A4成分蛋白）低聚物(SNCA oligomer)ELISA kit
 
 英文名称： Human synuclein, alpha (non A4 component of amyloid precursor) oligomer (SNCA oligomer) ELISA kit
 
 货号： CSB-E18033h
 
 规格： 96T
 
 种属： Human
 
 待测物名称： synuclein, alpha (non A4 component of amyloid precursor)
 
 缩写： SNCA
 
 反应时间： 1-5h
 
 所需样本体积： 50-100ul
 
 检测波长： 450 nm
 
 用途： For research use only. Not for diagnostic use.
 
 原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SNCA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SNCA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SNCA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SNCA bound in the initial step. The color development is stopped and the intensity of the color is measured. 
 特异性： This assay has high sensitivity and excellent specificity for detection of Human SNCA. No significant cross-reactivity or interference between Human SNCA and analogues was observed. 
 精密度： Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
 
 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the SNCA concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
 
相关产品：
 
产品名称 英文名称 货号 规格
 Rat Alpha-synuclein(SNCA) ELISA kit  Rat Alpha-synuclein(SNCA) ELISA kit  CSB-EL021912RA  96T 
        
 Pig Alpha-synuclein(SNCA) ELISA kit  Pig Alpha-synuclein(SNCA) ELISA kit  CSB-EL021912PI  96T 
        
 Mouse Alpha-synuclein(SNCA) ELISA kit  Mouse Alpha-synuclein(SNCA) ELISA kit  CSB-EL021912MO  96T 
        
 Monkey Alpha-synuclein(SNCA) ELISA kit  Monkey Alpha-synuclein(SNCA) ELISA kit  CSB-EL021912RH  96T 
        
 Bovine Alpha-synuclein(SNCA) ELISA kit  Bovine Alpha-synuclein(SNCA) ELISA kit  CSB-EL021912BO  96T 
        
抗体 
产品名称 货号 规格
 Rabbit anti-human synuclein, alpha (non A4 component of amyloid precursor) polyclonal Antibody  CSB-PA021912GA01HU  150ul 
      
蛋白 
产品名称 货号 规格 蛋白来源
 Recombinant Pan paniscus Alpha-synuclein(SNCA)  CSB-EP021912EQU  1mg  E.coli 
        
 Recombinant Pan paniscus Alpha-synuclein(SNCA)  CSB-YP021912EQU  1mg  Yeast 
        
 Recombinant Pan troglodytes Alpha-synuclein(SNCA)  CSB-EP021912EQV  1mg  E.coli 
        
 Recombinant Pan troglodytes Alpha-synuclein(SNCA)  CSB-YP021912EQV  1mg  Yeast 
        
 Recombinant Gorilla gorilla gorilla Alpha-synuclein(SNCA)  CSB-EP021912GGZ  1mg  E.coli 
        
 Recombinant Gorilla gorilla gorilla Alpha-synuclein(SNCA)  CSB-YP021912GGZ  1mg  Yeast 
        
 Recombinant Pongo abelii Alpha-synuclein(SNCA)  CSB-EP021912PYX  1mg  E.coli 
        
 Recombinant Pongo abelii Alpha-synuclein(SNCA)  CSB-YP021912PYX  1mg  Yeast 
        
 Recombinant Lagothrix lagotricha Alpha-synuclein(SNCA)  CSB-EP021912LNL  1mg  E.coli 
        
 Recombinant Lagothrix lagotricha Alpha-synuclein(SNCA)  CSB-YP021912LNL  1mg  Yeast 
        
 Recombinant Macaca fascicularis Alpha-synuclein(SNCA)  CSB-EP021912MOV  1mg  E.coli 
        
 Recombinant Macaca fascicularis Alpha-synuclein(SNCA)  CSB-YP021912MOV  1mg  Yeast 
        
 Recombinant Erythrocebus patas Alpha-synuclein(SNCA)  CSB-EP021912EGU  1mg  E.coli 
        
 Recombinant Erythrocebus patas Alpha-synuclein(SNCA)  CSB-YP021912EGU  1mg  Yeast 
        
 Recombinant Saguinus labiatus Alpha-synuclein(SNCA)  CSB-EP021912SVY  1mg  E.coli 
        
 Recombinant Saguinus labiatus Alpha-synuclein(SNCA)  CSB-YP021912SVY  1mg  Yeast 
        
 Recombinant Ateles geoffroyi Alpha-synuclein(SNCA)  CSB-EP021912AVF  1mg  E.coli 
        
 Recombinant Ateles geoffroyi Alpha-synuclein(SNCA)  CSB-YP021912AVF  1mg  Yeast 
        
 Recombinant Mouse Alpha-synuclein(Snca)  CSB-EP021912MO  1mg  E.coli 
        
 Recombinant Mouse Alpha-synuclein(Snca)  CSB-YP021912MO  1mg  Yeast 
        
 Recombinant Macaca mulatta (Rhesus macaque) Alpha-synuclein(SNCA)  CSB-EP021912  1mg  E.coli 
        
 Recombinant Macaca mulatta (Rhesus macaque) Alpha-synuclein(SNCA)  CSB-YP021912  1mg  Yeast 
        
 Recombinant Rat Alpha-synuclein(Snca)  CSB-EP021912RA  1mg  E.coli 
        
 Recombinant Rat Alpha-synuclein(Snca)  CSB-YP021912RA  1mg  Yeast 
        
 Recombinant Human Alpha-synuclein(SNCA)  CSB-EP021912HU  1mg  E.coli 
        
 Recombinant Human Alpha-synuclein(SNCA)  CSB-YP021912HU  1mg  Yeast