产品名称: 人纤维蛋白原α链(FGA)ELISA kit
英文名称: Human Fibrinogen alpha chain (FGA) ELISA kit
别名: Fib2, MGC119422, MGC119423, MGC119425, fibrinogen
货号: CSB-EL008607HU
规格: 96T
种属: Human
待测物名称: Fibrinogen alpha chain (FGA)
缩写: FGA
样本类型: serum, plasma, tissue homogenates
检测范围: 0.94μg/ml-60 μg/ml
灵敏度: 0.47 μg/ml
反应时间: 1-3h
所需样本体积: 50-100ul
检测波长: 450nm
用途: For research use only. Not for diagnostic use.
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGA bound in the initial step. The color development is stopped and the intensity of the color is measured.
特异性: This assay has high sensitivity and excellent specificity for detection of Human FGA. No significant cross-reactivity or interference between Human FGA and analogues was observed.
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the FGA concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
相关产品:
产品名称 英文名称 货号 规格
Monkey fibrinogen alpha chain (FGA) ELISA kit Monkey fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607RH 96T
Sheep fibrinogen alpha chain (FGA) ELISA kit Sheep fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607SH 96T
大鼠纤维蛋白原α链(Fga) ELISA Kit Rat fibrinogen alpha chain (Fga) ELISA kit CSB-E17440r 96T
Rabbit fibrinogen alpha chain (FGA) ELISA kit Rabbit fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607RB 96T
Pig fibrinogen alpha chain (FGA) ELISA kit Pig fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607PI 96T
Horse fibrinogen alpha chain (FGA) ELISA kit Horse fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607HO 96T
Guinea pig fibrinogen alpha chain (FGA) ELISA kit Guinea pig fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607GU 96T
Goat fibrinogen alpha chain (FGA) ELISA kit Goat fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607GO 96T
Donkey fibrinogen alpha chain (FGA) ELISA kit Donkey fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607DK 96T
Dog fibrinogen alpha chain (FGA) ELISA kit Dog fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607DO 96T
Chicken fibrinogen alpha chain (FGA) ELISA kit Chicken fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607CH 96T
Cat fibrinogen alpha chain (FGA) ELISA kit Cat fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607CA 96T
Bovine fibrinogen alpha chain (FGA) ELISA kit Bovine fibrinogen alpha chain (FGA) ELISA kit CSB-EL008607BO 96T
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Rat Fibrinogen alpha chain(Fga) CSB-EP008607RA 1mg E.coli
Recombinant Rat Fibrinogen alpha chain(Fga) CSB-YP008607RA 1mg Yeast
Recombinant Ceratotherium simum Fibrinogen alpha chain(FGA) CSB-EP008607DRG 1mg E.coli
Recombinant Ceratotherium simum Fibrinogen alpha chain(FGA) CSB-YP008607DRG 1mg Yeast
Recombinant Rangifer tarandus Fibrinogen alpha chain(FGA) CSB-EP008607RBL 1mg E.coli
Recombinant Rangifer tarandus Fibrinogen alpha chain(FGA) CSB-YP008607RBL 1mg Yeast
Recombinant Muntiacus muntjak Fibrinogen alpha chain(FGA) CSB-EP008607MJP 1mg E.coli
Recombinant Muntiacus muntjak Fibrinogen alpha chain(FGA) CSB-YP008607MJP 1mg Yeast
Recombinant Camelus dromedarius Fibrinogen alpha chain(FGA) CSB-EP008607CYV 1mg E.coli
Recombinant Camelus dromedarius Fibrinogen alpha chain(FGA) CSB-YP008607CYV 1mg Yeast
Recombinant Anas platyrhynchos Fibrinogen alpha chain(FGA) CSB-EP008607BZD 1mg E.coli
Recombinant Anas platyrhynchos Fibrinogen alpha chain(FGA) CSB-YP008607BZD 1mg Yeast
Recombinant Odocoileus hemionus Fibrinogen alpha chain(FGA) CSB-EP008607OAJ 1mg E.coli
Recombinant Odocoileus hemionus Fibrinogen alpha chain(FGA) CSB-YP008607OAJ 1mg Yeast
Recombinant Meles meles Fibrinogen alpha chain(FGA) CSB-EP008607MDP 1mg E.coli
Recombinant Meles meles Fibrinogen alpha chain(FGA) CSB-YP008607MDP 1mg Yeast
Recombinant Hylobates lar Fibrinogen alpha chain(FGA) CSB-EP008607HXW 1mg E.coli
Recombinant Hylobates lar Fibrinogen alpha chain(FGA) CSB-YP008607HXW 1mg Yeast
Recombinant Antilocapra americana Fibrinogen alpha chain(FGA) CSB-EP008607ALS 1mg E.coli
Recombinant Antilocapra americana Fibrinogen alpha chain(FGA) CSB-YP008607ALS 1mg Yeast
Recombinant Tapirus terrestris Fibrinogen alpha chain(FGA) CSB-EP008607TLG 1mg E.coli
Recombinant Tapirus terrestris Fibrinogen alpha chain(FGA) CSB-YP008607TLG 1mg Yeast
Recombinant Syncerus caffer Fibrinogen alpha chain(FGA) CSB-EP008607FPX 1mg E.coli
Recombinant Syncerus caffer Fibrinogen alpha chain(FGA) CSB-YP008607FPX 1mg Yeast
Recombinant Mandrillus leucophaeus Fibrinogen alpha chain(FGA) CSB-EP008607MBX 1mg E.coli
Recombinant Mandrillus leucophaeus Fibrinogen alpha chain(FGA) CSB-YP008607MBX 1mg Yeast
Recombinant Cervus nippon Fibrinogen alpha chain(FGA) CSB-EP008607CHC 1mg E.coli
Recombinant Cervus nippon Fibrinogen alpha chain(FGA) CSB-YP008607CHC 1mg Yeast
Recombinant Bison bonasus Fibrinogen alpha chain(FGA) CSB-EP008607BTF 1mg E.coli
Recombinant Bison bonasus Fibrinogen alpha chain(FGA) CSB-YP008607BTF 1mg Yeast
Recombinant Ammotragus lervia Fibrinogen alpha chain(FGA) CSB-EP008607BYG 1mg E.coli
Recombinant Ammotragus lervia Fibrinogen alpha chain(FGA) CSB-YP008607BYG 1mg Yeast
Recombinant Macaca fascicularis Fibrinogen alpha chain(FGA) CSB-EP008607MOV 1mg E.coli
Recombinant Macaca fascicularis Fibrinogen alpha chain(FGA) CSB-YP008607MOV 1mg Yeast
Recombinant Lama glama Fibrinogen alpha chain(FGA) CSB-EP008607LBS 1mg E.coli
Recombinant Lama glama Fibrinogen alpha chain(FGA) CSB-YP008607LBS 1mg Yeast
Recombinant Vulpes vulpes Fibrinogen alpha chain(FGA) CSB-EP008607VFW 1mg E.coli
Recombinant Vulpes vulpes Fibrinogen alpha chain(FGA) CSB-YP008607VFW 1mg Yeast
Recombinant Theropithecus gelada Fibrinogen alpha chain(FGA) CSB-EP008607TNU 1mg E.coli
Recombinant Theropithecus gelada Fibrinogen alpha chain(FGA) CSB-YP008607TNU 1mg Yeast
Recombinant Erythrocebus patas Fibrinogen alpha chain(FGA) CSB-EP008607EGU 1mg E.coli
Recombinant Erythrocebus patas Fibrinogen alpha chain(FGA) CSB-YP008607EGU 1mg Yeast
Recombinant Lama vicugna Fibrinogen alpha chain(FGA) CSB-EP008607LBV 1mg E.coli
Recombinant Lama vicugna Fibrinogen alpha chain(FGA) CSB-YP008607LBV 1mg Yeast
Recombinant Papio anubis Fibrinogen alpha chain(FGA) CSB-EP008607ERI 1mg E.coli
Recombinant Papio anubis Fibrinogen alpha chain(FGA) CSB-YP008607ERI 1mg Yeast
Recombinant Cervus elaphus nelsoni Fibrinogen alpha chain(FGA) CSB-EP008607CHB 1mg E.coli
Recombinant Cervus elaphus nelsoni Fibrinogen alpha chain(FGA) CSB-YP008607CHB 1mg Yeast
Recombinant Papio hamadryas Fibrinogen alpha chain(FGA) CSB-EP008607ERJ 1mg E.coli
Recombinant Papio hamadryas Fibrinogen alpha chain(FGA) CSB-YP008607ERJ 1mg Yeast
Recombinant Macaca fuscata fuscata Fibrinogen alpha chain(FGA) CSB-EP008607MMX 1mg E.coli
Recombinant Macaca fuscata fuscata Fibrinogen alpha chain(FGA) CSB-YP008607MMX 1mg Yeast
Recombinant Cervus elaphus Fibrinogen alpha chain(FGA) CSB-EP008607DRN 1mg E.coli
Recombinant Cervus elaphus Fibrinogen alpha chain(FGA) CSB-YP008607DRN 1mg Yeast
Recombinant Chlorocebus aethiops Fibrinogen alpha chain(FGA) CSB-EP008607DSU 1mg E.coli
Recombinant Chlorocebus aethiops Fibrinogen alpha chain(FGA) CSB-YP008607DSU 1mg Yeast
Recombinant Bovine Fibrinogen alpha chain(FGA) CSB-EP008607BO 1mg E.coli
Recombinant Bovine Fibrinogen alpha chain(FGA) CSB-YP008607BO 1mg Yeast
Recombinant Human Fibrinogen alpha chain(FGA) CSB-EP008607HU 1mg E.coli
Recombinant Human Fibrinogen alpha chain(FGA) CSB-YP008607HU 1mg Yeast
Recombinant Horse Fibrinogen alpha chain(FGA) CSB-EP008607HO 1mg E.coli
Recombinant Horse Fibrinogen alpha chain(FGA) CSB-YP008607HO 1mg Yeast
Recombinant Chicken Fibrinogen alpha chain(FGA) CSB-EP008607CH 1mg E.coli
Recombinant Chicken Fibrinogen alpha chain(FGA) CSB-YP008607CH 1mg Yeast
Recombinant Pig Fibrinogen alpha chain(FGA) CSB-EP008607PI 1mg E.coli
Recombinant Pig Fibrinogen alpha chain(FGA) CSB-YP008607PI 1mg Yeast
Recombinant Donkey Fibrinogen alpha chain(FGA) CSB-EP008607DK 1mg E.coli
Recombinant Donkey Fibrinogen alpha chain(FGA) CSB-YP008607DK 1mg Yeast
Recombinant Guinea pig Fibrinogen alpha chain(FGA) CSB-EP008607GU 1mg E.coli
Recombinant Guinea pig Fibrinogen alpha chain(FGA) CSB-YP008607GU 1mg Yeast
Recombinant Rabbit Fibrinogen alpha chain(FGA) CSB-EP008607RB 1mg E.coli
Recombinant Rabbit Fibrinogen alpha chain(FGA) CSB-YP008607RB 1mg Yeast
Recombinant Sheep Fibrinogen alpha chain(FGA) CSB-EP008607SH 1mg E.coli
Recombinant Sheep Fibrinogen alpha chain(FGA) CSB-YP008607SH 1mg Yeast
Recombinant Dog Fibrinogen alpha chain(FGA) CSB-EP008607DO 1mg E.coli
Recombinant Dog Fibrinogen alpha chain(FGA) CSB-YP008607DO 1mg Yeast
Recombinant Macaca mulatta (Rhesus macaque) Fibrinogen alpha chain(FGA) CSB-EP008607 1mg E.coli
Recombinant Macaca mulatta (Rhesus macaque) Fibrinogen alpha chain(FGA) CSB-YP008607 1mg Yeast
Recombinant Goat Fibrinogen alpha chain(FGA) CSB-EP008607GO 1mg E.coli
Recombinant Goat Fibrinogen alpha chain(FGA) CSB-YP008607GO 1mg Yeast
人纤维蛋白原α链(FGA)ELISA Kit 由上海希美化学有限公司为您提供,货号CSB-EL008607HU,规格:96T,CAS号:CSB-EL008607HU,如您想了解更多关于人纤维蛋白原α链(FGA)ELISA Kit 价格、人纤维蛋白原α链(FGA)ELISA Kit 结构式、批发、用途等信息,欢迎咨询。除供应人纤维蛋白原α链(FGA)ELISA Kit 外,还可为您提供兔血纤肽/纤维蛋白肽A(FPA)ELISA Kit 、兔子纤溶酶原(Plg)ELISA Kit 、大鼠纤维蛋白原(Fb)ELISA Kit等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,上海希美化学客户服务电话,售前、售后均可联系。