产品名称: 人TATA盒结合蛋白/TBP相关因子(TAF)ELISA Kit
英文名称: Human TATA box binding protein/TBP-associated factors,TAF ELISA Kit
别名: GTF2D, GTF2D1, MGC117320, MGC126054, MGC126055, SCA17, TFIID, TATA-box binding protein N-terminal domain
货号: CSB-E04995h
规格: 96T
种属: Human
待测物名称: TATA box binding protein
缩写: TBP
蛋白功能1: Immunity
蛋白功能3: Host-virus interaction
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
用途: For research use only. Not for diagnostic use.
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
特异性: This assay has high sensitivity and excellent specificity for detection of Human TBP. No significant cross-reactivity or interference between Human TBP and analogues was observed.
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the TBP concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
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Recombinant Cenarchaeum symbiosum TATA-box-binding protein(tbp) CSB-YP023239DRA 1mg Yeast
Recombinant Methanothermobacter thermautotrophicus TATA-box-binding protein(tbp) CSB-EP023239MSR 1mg E.coli
Recombinant Methanothermobacter thermautotrophicus TATA-box-binding protein(tbp) CSB-YP023239MSR 1mg Yeast
Recombinant Archaeoglobus fulgidus TATA-box-binding protein(tbp) CSB-EP023239DOC 1mg E.coli
Recombinant Archaeoglobus fulgidus TATA-box-binding protein(tbp) CSB-YP023239DOC 1mg Yeast
Recombinant Artemia franciscana TATA-box-binding protein CSB-EP023239DOF 1mg E.coli
Recombinant Artemia franciscana TATA-box-binding protein CSB-YP023239DOF 1mg Yeast
Recombinant Thermococcus sibiricus TATA-box-binding protein(tbp) CSB-EP023239TMT 1mg E.coli
Recombinant Thermococcus sibiricus TATA-box-binding protein(tbp) CSB-YP023239TMT 1mg Yeast
Recombinant Thermococcus onnurineus TATA-box-binding protein(tbp) CSB-EP023239TMR 1mg E.coli
Recombinant Thermococcus onnurineus TATA-box-binding protein(tbp) CSB-YP023239TMR 1mg Yeast
Recombinant Thermoproteus neutrophilus TATA-box-binding protein(tbp) CSB-EP023239TNE 1mg E.coli
Recombinant Thermoproteus neutrophilus TATA-box-binding protein(tbp) CSB-YP023239TNE 1mg Yeast
Recombinant Methanococcus maripaludis TATA-box-binding protein(tbp) CSB-EP023239MNQ 1mg E.coli
Recombinant Methanococcus maripaludis TATA-box-binding protein(tbp) CSB-YP023239MNQ 1mg Yeast
Recombinant Methanococcus vannielii TATA-box-binding protein(tbp) CSB-EP023239MNS 1mg E.coli
Recombinant Methanococcus vannielii TATA-box-binding protein(tbp) CSB-YP023239MNS 1mg Yeast
Recombinant Methanococcus aeolicus TATA-box-binding protein(tbp) CSB-EP023239MNO 1mg E.coli
Recombinant Methanococcus aeolicus TATA-box-binding protein(tbp) CSB-YP023239MNO 1mg Yeast
Recombinant Methanococcus maripaludis TATA-box-binding protein(tbp) CSB-EP023239MNR 1mg E.coli
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Recombinant Methanobrevibacter smithii TATA-box-binding protein(tbp) CSB-EP023239MNN 1mg E.coli
Recombinant Methanobrevibacter smithii TATA-box-binding protein(tbp) CSB-YP023239MNN 1mg Yeast
Recombinant Pyrobaculum calidifontis TATA-box-binding protein(tbp) CSB-EP023239PZV 1mg E.coli
Recombinant Pyrobaculum calidifontis TATA-box-binding protein(tbp) CSB-YP023239PZV 1mg Yeast
Recombinant Pyrobaculum islandicum TATA-box-binding protein(tbp) CSB-EP023239PZW 1mg E.coli
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Recombinant Methanosaeta thermophila TATA-box-binding protein(tbp) CSB-EP023239MNW 1mg E.coli
Recombinant Methanosaeta thermophila TATA-box-binding protein(tbp) CSB-YP023239MNW 1mg Yeast
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Recombinant TATA-box-binding protein(tbp) CSB-YP023239FHZ 1mg Yeast
Recombinant Pyrococcus furiosus TATA-box-binding protein(tbp) CSB-EP023239FHW 1mg E.coli
Recombinant Pyrococcus furiosus TATA-box-binding protein(tbp) CSB-YP023239FHW 1mg Yeast
Recombinant Sulfolobus solfataricus TATA-box-binding protein(tbp) CSB-EP023239FPM 1mg E.coli
Recombinant Sulfolobus solfataricus TATA-box-binding protein(tbp) CSB-YP023239FPM 1mg Yeast
Recombinant TATA-box-binding protein(tbp) CSB-EP023239PXU 1mg E.coli
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Recombinant Spodoptera frugiperda TATA-box-binding protein(Tbp) CSB-EP023239FKQ 1mg E.coli
Recombinant Spodoptera frugiperda TATA-box-binding protein(Tbp) CSB-YP023239FKQ 1mg Yeast
Recombinant Acetabularia cliftonii TATA-box-binding protein CSB-EP023239AAW 1mg E.coli
Recombinant Acetabularia cliftonii TATA-box-binding protein CSB-YP023239AAW 1mg Yeast
Recombinant Mesembryanthemum crystallinum TATA-box-binding protein CSB-EP023239MRC 1mg E.coli
Recombinant Mesembryanthemum crystallinum TATA-box-binding protein CSB-YP023239MRC 1mg Yeast
Recombinant TATA-box-binding protein(tbp-1) CSB-EP023239CXY 1mg E.coli
Recombinant TATA-box-binding protein(tbp-1) CSB-YP023239CXY 1mg Yeast
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Recombinant TATA-box-binding protein(TBP) CSB-YP023239PLO 1mg Yeast
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Recombinant Acanthamoeba castellanii TATA-box-binding protein(TFIID) CSB-YP023239AAJ 1mg Yeast
Recombinant Solanum tuberosum TATA-box-binding protein(TBP) CSB-EP023239FIG 1mg E.coli
Recombinant Solanum tuberosum TATA-box-binding protein(TBP) CSB-YP023239FIG 1mg Yeast
Recombinant Xenopus laevis TATA-box-binding protein(tbp) CSB-EP023239XBE 1mg E.coli
Recombinant Xenopus laevis TATA-box-binding protein(tbp) CSB-YP023239XBE 1mg Yeast
Recombinant Dictyostelium discoideum TATA-box-binding protein(tbpA) CSB-EP023239DKK 1mg E.coli
Recombinant Dictyostelium discoideum TATA-box-binding protein(tbpA) CSB-YP023239DKK 1mg Yeast
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Recombinant Drosophila melanogaster TATA-box-binding protein(Tbp) CSB-YP023239DLU 1mg Yeast
Recombinant Saccharomyces cerevisiae TATA-box-binding protein(SPT15) CSB-EP023239SVG 1mg E.coli
Recombinant Saccharomyces cerevisiae TATA-box-binding protein(SPT15) CSB-YP023239SVG 1mg Yeast
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Recombinant Nicotiana tabacum TATA-box-binding protein CSB-YP023239NHE 1mg Yeast
Recombinant Chicken TATA-box-binding protein(TBP) CSB-EP023239CH 1mg E.coli
Recombinant Chicken TATA-box-binding protein(TBP) CSB-YP023239CH 1mg Yeast
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Recombinant Mouse TATA-box-binding protein(Tbp) CSB-YP023239MO 1mg Yeast
Recombinant Human TATA-box-binding protein(TBP) CSB-EP023239HU 1mg E.coli
Recombinant Human TATA-box-binding protein(TBP) CSB-YP023239HU 1mg Yeast
人TATA盒结合蛋白/TBP相关因子(TAF)ELISA Kit 由上海希美化学有限公司为您提供,货号CSB-E04995h,规格:96T,CAS号:CSB-E04995h,如您想了解更多关于人TATA盒结合蛋白/TBP相关因子(TAF)ELISA Kit 价格、人TATA盒结合蛋白/TBP相关因子(TAF)ELISA Kit 结构式、批发、用途等信息,欢迎咨询。除供应人TATA盒结合蛋白/TBP相关因子(TAF)ELISA Kit 外,还可为您提供小鼠纤溶抑制因子/凝血酶激活的纤溶抑制物(TAFI)ELISA Kit等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,上海希美化学客户服务电话,售前、售后均可联系。