产品名称: 山羊胰岛素(INS)ELISA Kit
英文名称: Goat Insulin(INS)ELISA Kit
货号: CSB-E17653G
价格: 3800
规格: 96T
种属: Goat
待测物名称: Insulin(INS)
缩写: INS
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
用途: For research use only. Not for diagnostic use.
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INS bound in the initial step. The color development is stopped and the intensity of the color is measured.
特异性: This assay has high sensitivity and excellent specificity for detection of Goat INS. No significant cross-reactivity or interference between Goat INS and analogues was observed.
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the INS concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
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抗体
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Rabbit anti-human Insulin(INS) polyclonal Antibody CSB-PA011742GA01HU 150ul
蛋白
产品名称 货号 规格 蛋白来源
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Recombinant Cyprinus carpio Insulin(ins) 1mg
Recombinant Proechimys guairae Insulin(INS) 1mg
Recombinant Chinchilla chinchilla Insulin(INS) 1mg
Recombinant Acomys cahirinus Insulin(INS) 1mg
Recombinant Platichthys flesus Insulin(ins) 1mg
Recombinant Oncorhynchus keta Insulin(ins) 1mg
Recombinant Katsuwonus pelamis Insulin(ins) 1mg
Recombinant Crotalus atrox Insulin(INS) 1mg
Recombinant Myocastor coypus Insulin(INS) 1mg
Recombinant Camelus dromedarius Insulin(INS) 1mg
Recombinant Balaenoptera borealis Insulin(INS) 1mg
Recombinant Atractosteus spatula Insulin(ins) 1mg
Recombinant Myoxocephalus scorpius Insulin(ins) 1mg
Recombinant Thunnus thynnus Insulin(ins) 1mg
Recombinant Gadus morhua subsp. callarias Insulin(ins) 1mg
Recombinant Anas platyrhynchos Insulin(INS) 1mg
Recombinant Hystrix cristata Insulin(INS) 1mg
Recombinant Elephas maximus Insulin(INS) 1mg
Recombinant Cricetulus longicaudatus Insulin(INS) 1mg
Recombinant Selasphorus rufus Insulin(INS) 1mg
Recombinant Anguilla anguilla Insulin(ins) 1mg
Recombinant Chlorocebus aethiops Insulin(INS) 1mg
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更多
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企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
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