小鼠瘦素(LEP)ELISA Kit

 产品名称： 小鼠瘦素(LEP)ELISA Kit
 
 英文名称： Mouse Leptin,LEP ELISA kit
 
 货号： CSB-E04650m
 
 价格： 3800
 
 规格： 96T
 
 种属： Mouse
 
 待测物名称： leptin
 
 缩写： LEP
 
 样本类型： serum, plasma, tissue homogenates and cell culture supernates
 
 检测范围： 15.6 pg/ml-1000 pg/ml
 
 灵敏度： 3.9 pg/ml
 
 反应时间： 1-5h
 
 所需样本体积： 50-100ul
 
 检测波长： 450 nm
 
 用途： For research use only. Not for diagnostic use.
 
 原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LEP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEP bound in the initial step. The color development is stopped and the intensity of the color is measured. 
 特异性： This assay has high sensitivity and excellent specificity for detection of Mouse LEP. No significant cross-reactivity or interference between Mouse LEP and analogues was observed. 
 精密度： Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
 
 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the LEP concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
 
相关产品：
 
产品名称 英文名称 货号 规格
 Camel Leptin(LEP) ELISA kit  Camel Leptin(LEP) ELISA kit  CSB-EL012870CM  96T 
        
 鸡瘦素(LEP)ELISA kit  Chicken Leptin,LEP ELISA kit  CSB-E14082C  96T 
        
 人瘦素(LEP)ELISA Kit  Human Leptin,LEP ELISA kit  CSB-E04649h  96T 
        
 鸭瘦素(LEP)ELISA kit  Duck Leptin,LEP ELISA kit  CSB-EL012870DU  96T 
        
 猴子瘦素(LEP)ELISA Kit  Monkey Leptin,LEP ELISA Kit  CSB-E14936Mk  96T 
        
 鱼(鲑鱼)瘦素(LEP) ELISA kit  Fish (Salmon) leptin (LEP) ELISA kit  CSB-EL012870FI  96T 
        
 猪瘦素(LEP)ELISA Kit  Pig Leptin,LEP ELISA Kit  CSB-E06815p  96T 
        
 牛瘦素(LEP)ELISA Kit  Bovine Leptin,LEP ELISA Kit  CSB-E06771b  96T 
        
 大鼠瘦素(LEP)ELISA kit  Rat Leptin,LEP ELISA kit  CSB-E07433r  96T 
        
 仓鼠瘦素(LEP) ELISA kit  Hamster leptin (LEP) ELISA kit  CSB-EL012870HA  96T 
        
 小尾寒羊瘦素(LEP)ELISA kit  Small Tailed Han Sheep Leptin,LEP ELISA kit  CSB-E12839Sh  96T 
        
 Sheep Leptin(LEP) ELISA kit  Sheep Leptin(LEP) ELISA kit  CSB-EL012870SH  96T 
        
 兔子瘦素(LEP)ELISA Kit  rabbit Leptin,LEP ELISA Kit  CSB-E06971Rb  96T 
        
 Horse Leptin(LEP) ELISA kit  Horse Leptin(LEP) ELISA kit  CSB-EL012870HO  96T 
        
 山羊瘦素(LEP)ELISA kit  Goat Leptin,LEP ELISA kit  CSB-E12834G  96T 
        
 Dog Leptin(LEP) ELISA kit  Dog Leptin(LEP) ELISA kit  CSB-EL012870DO  96T 
        
 Cat Leptin(LEP) ELISA kit  Cat Leptin(LEP) ELISA kit  CSB-EL012870CA  96T 
        
抗体 
产品名称 货号 规格
 Rabbit anti-human Leptin,LEP polyclonal Antibody  CSB-PA012870GA01HU  150ul 
      
蛋白 
产品名称 货号 规格 蛋白来源
 Recombinant Takifugu rubripes Leptin(lep)  1mg 
        
 Recombinant Pig Leptin(LEP)  1mg 
        
 Recombinant Ursus thibetanus Leptin(LEP)  1mg 
        
 Recombinant Macaca mulatta Leptin(LEP)  1mg 
        
 Recombinant Goat Leptin(LEP)  1mg 
        
 Recombinant Sheep Leptin(LEP)  1mg 
        
 Recombinant Sminthopsis crassicaudata Leptin(LEP)  1mg 
        
 Recombinant Horse Leptin(LEP)  1mg 
        
 Recombinant Cat Leptin(LEP)  1mg 
        
 Recombinant Gorilla gorilla gorilla Leptin(LEP)  1mg 
        
 Recombinant Pongo pygmaeus Leptin(LEP)  1mg 
        
 Recombinant Halichoerus grypus Leptin(LEP)  1mg 
        
 Recombinant Phoca vitulina Leptin(LEP)  1mg 
        
 Recombinant Bubalus bubalis Leptin(LEP)  1mg 
        
 Recombinant Tetraodon nigroviridis Leptin(lep)  1mg 
        
 Recombinant Meleagris gallopavo Leptin(LEP)  1mg 
        
 Recombinant Pan troglodytes Leptin(LEP)  1mg 
        
 Recombinant Dog Leptin(LEP)  1mg 
        
 Recombinant Chicken Leptin(LEP)  1mg 
        
 Recombinant Rat Leptin(Lep)  1mg 
        
 Recombinant Bovine Leptin(LEP)  1mg 
        
 Recombinant Human Leptin(LEP)  1mg 
        
 Recombinant Mouse Leptin(Lep)  1mg