小鼠细胞色素C(Cyt-C)ELISA Kit

 产品名称： 小鼠细胞色素C(Cyt-C)ELISA Kit
 
 英文名称： Mouse Cytochrome-C,Cyt-C ELISA Kit
 
 别名： CYC, HCS, THC4
 
 货号： CSB-E08532m
 
 规格： 96T
 
 种属： Mouse
 
 待测物名称： cytochrome c, somatic
 
 缩写： CYCS
 
 样本类型： serum, plasma, tissue homogenates, cell lysates
 
 检测范围： 34.3 pg/ml-2200 pg/ml
 
 灵敏度： 8.5 pg/ml
 
 反应时间： 1-5h
 
 所需样本体积： 50-100ul
 
 检测波长： 450 nm
 
 用途： For research use only. Not for diagnostic use.
 
 原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYCS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYCS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYCS bound in the initial step. The color development is stopped and the intensity of the color is measured. 
 特异性： This assay has high sensitivity and excellent specificity for detection of Mouse CYCS. No significant cross-reactivity or interference between Mouse CYCS and analogues was observed. 
 精密度： Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
 
 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the CYCS concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
 
相关产品：
 
产品名称 英文名称 货号 规格
 Pig Cytochrome c(CYCS) ELISA kit  Pig Cytochrome c(CYCS) ELISA kit  CSB-EL006328PI  96T 
        
 Rat Cytochrome c(CYCS) ELISA kit  Rat Cytochrome c(CYCS) ELISA kit  CSB-EL006328RA  96T 
        
 人细胞色素C(Cyt-C)ELISA Kit  Human Cytochrome-C,Cyt-C ELISA Kit  CSB-E08530h  96T 
        
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 Rabbit Cytochrome c(CYCS) ELISA kit  Rabbit Cytochrome c(CYCS) ELISA kit  CSB-EL006328RB  96T 
        
 Monkey Cytochrome c(CYCS) ELISA kit  Monkey Cytochrome c(CYCS) ELISA kit  CSB-EL006328RH  96T 
        
 Horse Cytochrome c(CYCS) ELISA kit  Horse Cytochrome c(CYCS) ELISA kit  CSB-EL006328HO  96T 
        
 Donkey Cytochrome c(CYCS) ELISA kit  Donkey Cytochrome c(CYCS) ELISA kit  CSB-EL006328DK  96T 
        
 Dog Cytochrome c(CYCS) ELISA kit  Dog Cytochrome c(CYCS) ELISA kit  CSB-EL006328DO  96T 
        
 Bovine Cytochrome c(CYCS) ELISA kit  Bovine Cytochrome c(CYCS) ELISA kit  CSB-EL006328BO  96T 
        
抗体 
产品名称 货号 规格
 Rabbit anti-human cytochrome c, somatic polyclonal Antibody  CSB-PA006328GA01HU  150ul 
      
蛋白 
产品名称 货号 规格 蛋白来源
 Recombinant Xenopus tropicalis Cytochrome c, somatic(cycs)  1mg 
        
 Recombinant Antirrhinum majus Transcription factor CYCLOIDEA(CYC)  1mg 
        
 Recombinant Drosophila melanogaster Protein cycle(cyc)  1mg 
        
 Recombinant Chlorobaculum parvum Photosynthetic reaction center cytochrome c-551(pscC)  1mg 
        
 Recombinant Escherichia coli D-serine/D-alanine/glycine transporter(cycA)  1mg 
        
 Recombinant D-serine/D-alanine/glycine transporter(cycA)  1mg 
        
 Recombinant Drosophila melanogaster G2/mitotic-specific cyclin-A(CycA)  1mg 
        
 Recombinant Pachysolen tannophilus Cytochrome c(CYC1)  1mg 
        
 Recombinant Fritillaria agrestis Cytochrome c(CYTC)  1mg 
        
 Recombinant Scheffersomyces stipitis Cytochrome c(CYC1)  1mg 
        
 Recombinant Chlorobium tepidum Cytochrome c(pscC)  1mg 
        
 Recombinant Otolemur garnettii Cytochrome c(CYCS)  1mg 
        
 Recombinant Oryza sativa subsp. indica Cytochrome c(CC-1)  1mg 
        
 Recombinant Cytochrome c  1mg 
        
 Recombinant Enteromorpha intestinalis Cytochrome c  1mg 
        
 Recombinant Fagopyrum esculentum Cytochrome c  1mg 
        
 Recombinant Triticum aestivum Cytochrome c  1mg 
        
 Recombinant Solanum tuberosum Cytochrome c  1mg 
        
 Recombinant Ricinus communis Cytochrome c  1mg 
        
 Recombinant Cannabis sativa Cytochrome c  1mg 
        
 Recombinant Ustilago sphaerogena Cytochrome c  1mg 
        
 Recombinant Issatchenkia orientalis Cytochrome c(CYC1)  1mg 
        
 Recombinant Apis mellifera Cytochrome c(cytC)  1mg 
        
 Recombinant Macrobrachium malcolmsonii Cytochrome c  1mg 
        
 Recombinant Squalus suckleyi Cytochrome c(cyc)  1mg 
        
 Recombinant Anas platyrhynchos Cytochrome c(CYC)  1mg 
        
 Recombinant Aptenodytes patagonicus Cytochrome c(CYC)  1mg 
        
 Recombinant Mirounga leonina Cytochrome c(CYCS)  1mg 
        
 Recombinant Hippopotamus amphibius Cytochrome c(CYCS)  1mg 
        
 Recombinant Cytochrome c  1mg 
        
 Recombinant Pastinaca sativa Cytochrome c  1mg 
        
 Recombinant Guizotia abyssinica Cytochrome c  1mg 
        
 Recombinant Nigella damascena Cytochrome c  1mg 
        
 Recombinant Acer negundo Cytochrome c  1mg 
        
 Recombinant Abutilon theophrasti Cytochrome c  1mg 
        
 Recombinant Sesamum indicum Cytochrome c  1mg 
        
 Recombinant Neurospora crassa Cytochrome c(cyc-1)  1mg 
        
 Recombinant Schizosaccharomyces pombe Cytochrome c(cyc1)  1mg 
        
 Recombinant Hansenula anomala Cytochrome c  1mg 
        
 Recombinant Haematobia irritans Cytochrome c  1mg 
        
 Recombinant Lampetra tridentata Cytochrome c(cyc)  1mg 
        
 Recombinant Lithobates catesbeiana Cytochrome c  1mg 
        
 Recombinant Struthio camelus Cytochrome c(CYC)  1mg 
        
 Recombinant Macropus giganteus Cytochrome c(CYCS)  1mg 
        
 Recombinant Ateles sp. Cytochrome c(CYCS)  1mg 
        
 Recombinant Cytochrome c  1mg 
        
 Recombinant Ginkgo biloba Cytochrome c  1mg 
        
 Recombinant Helianthus annuus Cytochrome c(CYTC1)  1mg 
        
 Recombinant Arum maculatum Cytochrome c  1mg 
        
 Recombinant Sambucus nigra Cytochrome c  1mg 
        
 Recombinant Gossypium barbadense Cytochrome c  1mg 
        
 Recombinant Vigna radiata var. radiata Cytochrome c  1mg 
        
 Recombinant Schistocerca gregaria Cytochrome c  1mg 
        
 Recombinant Manduca sexta Cytochrome c  1mg 
        
 Recombinant Helix aspersa Cytochrome c  1mg 
        
 Recombinant Asterias rubens Cytochrome c  1mg 
        
 Recombinant Katsuwonus pelamis Cytochrome c(cyc)  1mg 
        
 Recombinant Chelydra serpentina Cytochrome c  1mg 
        
 Recombinant Dromaius novaehollandiae Cytochrome c(CYC)  1mg 
        
 Recombinant Miniopterus schreibersii Cytochrome c(CYCS)  1mg 
        
 Recombinant Cytochrome c  1mg 
        
 Recombinant Spinacia oleracea Cytochrome c  1mg 
        
 Recombinant Tropaeolum majus Cytochrome c  1mg 
        
 Recombinant Allium porrum Cytochrome c  1mg