大鼠硫氧化还原蛋白(Trx)ELISA Kit

 产品名称： 大鼠硫氧化还原蛋白(Trx)ELISA Kit
 
 英文名称： Rat Thioredoxin,Trx ELISA Kit
 
 货号： CSB-E09729r
 
 规格： 96T
 
 种属： Rat
 
 待测物名称： thioredoxin
 
 缩写： TXN
 
 蛋白功能1： Transport
 
 蛋白功能2： Anion transport
 
 蛋白功能3： Electron transport
 
 反应时间： 1-5h
 
 所需样本体积： 50-100ul
 
 检测波长： 450 nm
 
 大鼠硫氧化还原蛋白(Trx)ELISA Kit

用途： For research use only. Not for diagnostic use.
 
 大鼠硫氧化还原蛋白(Trx)ELISA Kit

原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TXN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TXN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TXN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TXN bound in the initial step. The color development is stopped and the intensity of the color is measured. 
 大鼠硫氧化还原蛋白(Trx)ELISA Kit

特异性： This assay has high sensitivity and excellent specificity for detection of Rat TXN. No significant cross-reactivity or interference between Rat TXN and analogues was observed. 
 大鼠硫氧化还原蛋白(Trx)ELISA Kit

精密度： Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 大鼠硫氧化还原蛋白(Trx)ELISA Kit

样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4&deg;C before centrifugation for 15 minutes at 1000 &times;g. Remove serum and assay immediately or aliquot and store samples at -20&deg;C or -80&deg;C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 &times;g at 2-8&deg;C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20&deg;C or -80&deg;C. Avoid repeated freeze-thaw cycles.
 
 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4&deg;C.
3. Add 100&mu;l of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37&deg;C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100&mu;l of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37&deg;C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200&mu;l) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100&mu;l of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37&deg;C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90&mu;l of TMB Substrate to each well. Incubate for 15-30 minutes at 37&deg;C. Protect from light.
10. Add 50&mu;l of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 大鼠硫氧化还原蛋白(Trx)ELISA Kit

结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the TXN concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
 
 大鼠硫氧化还原蛋白(Trx)ELISA Kit  
产品名称 英文名称 货号 规格
 小鼠硫氧化还原蛋白(Trx)ELISA Kit  Mouse Thioredoxin,Trx ELISA Kit  CSB-E09730m  96T 
        
 Sheep Thioredoxin(TXN) ELISA kit  Sheep Thioredoxin(TXN) ELISA kit  CSB-EL025365SH  96T 
        
 Rabbit Thioredoxin(TXN) ELISA kit  Rabbit Thioredoxin(TXN) ELISA kit  CSB-EL025365RB  96T 
        
 Pig Thioredoxin(TXN) ELISA kit  Pig Thioredoxin(TXN) ELISA kit  CSB-EL025365PI  96T 
        
 Monkey Thioredoxin(TXN) ELISA kit  Monkey Thioredoxin(TXN) ELISA kit  CSB-EL025365RH  96T 
        
 人硫氧化还原蛋白(Trx)ELISA Kit  Human Thioredoxin,Trx ELISA Kit  CSB-E09728h  96T 
        
 Horse Thioredoxin(TXN) ELISA kit  Horse Thioredoxin(TXN) ELISA kit  CSB-EL025365HO  96T 
        
 Dog Thioredoxin(TXN) ELISA kit  Dog Thioredoxin(TXN) ELISA kit  CSB-EL025365DO  96T 
        
 Chicken Thioredoxin(TXN) ELISA kit  Chicken Thioredoxin(TXN) ELISA kit  CSB-EL025365CH  96T 
        
 Bovine Thioredoxin(TXN) ELISA kit  Bovine Thioredoxin(TXN) ELISA kit  CSB-EL025365BO  96T 
        
大鼠硫氧化还原蛋白(Trx)ELISA Kit

抗体 
产品名称 货号 规格
 Rabbit anti-human thioredoxin polyclonal Antibody  CSB-PA025365GA01HU  150ul 
      
大鼠硫氧化还原蛋白(Trx)ELISA Kit

蛋白 
产品名称 货号 规格 蛋白来源
 Recombinant Staphylococcus aureus Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus epidermidis Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus saprophyticus subsp. saprophyticus Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus aureus Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus aureus Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus aureus Thioredoxin(trxA)  1mg 
        
 Recombinant Rickettsia bellii Thioredoxin(trxA)  1mg 
        
 Recombinant Rickettsia prowazekii Thioredoxin(trxA)  1mg 
        
 Recombinant Chlamydia pneumoniae Thioredoxin(trxA)  1mg 
        
 Recombinant Pseudomonas aeruginosa Thioredoxin(trxA)  1mg 
        
 Recombinant Coprinus comatus Thioredoxin  1mg 
        
 Recombinant Mycoplasma gallisepticum Thioredoxin(trxA)  1mg 
        
 Recombinant Chlamydia muridarum Thioredoxin(trxA)  1mg 
        
 Recombinant Ictalurus punctatus Thioredoxin(txn)  1mg 
        
 Recombinant Pasteurella multocida Thioredoxin(trxA)  1mg 
        
 Recombinant Ophiophagus hannah Thioredoxin(TXN)  1mg 
        
 Recombinant Callithrix jacchus Thioredoxin(TXN)  1mg 
        
 Recombinant Mycoplasma pulmonis Thioredoxin(trxA)  1mg 
        
 Recombinant Rickettsia conorii Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus epidermidis Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus aureus Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus aureus Thioredoxin(trxA)  1mg 
        
 Recombinant Chloroflexus aurantiacus Thioredoxin(trxA)  1mg 
        
 Recombinant Plasmodium falciparum Thioredoxin(PF14_0545)  1mg 
        
 Recombinant Rickettsia typhi Thioredoxin(trxA)  1mg 
        
 Recombinant Pongo abelii Thioredoxin(TXN)  1mg 
        
 Recombinant Methanocaldococcus jannaschii Thioredoxin(trx)  1mg 
        
 Recombinant Rickettsia felis Thioredoxin(trxA)  1mg 
        
 Recombinant Bacillus subtilis Glycine oxidase(thiO)  1mg 
        
 Recombinant Rhizobium etli Putative thiamine biosynthesis oxidoreductase thiO(thiO)  1mg 
        
 Recombinant Schizosaccharomyces pombe Thioredoxin-1(trx1)  1mg 
        
 Recombinant Salmonella typhimurium Thioredoxin-1(trxA)  1mg 
        
 Recombinant Thioredoxin-1(trxA)  1mg 
        
 Recombinant Anabaena sp. Thioredoxin-1(trxA)  1mg 
        
 Recombinant Thioredoxin-1(trxA)  1mg 
        
 Recombinant Thioredoxin-1(trxA)  1mg 
        
 Recombinant Thioredoxin-1(trxA)  1mg 
        
 Recombinant Escherichia coli Thioredoxin-1(trxA)  1mg 
        
 Recombinant Nostoc sp. Thioredoxin-1(trxA)  1mg 
        
 Recombinant Dictyostelium discoideum Thioredoxin-1(trxA)  1mg 
        
 Recombinant Arabidopsis thaliana Thioredoxin H1(TRX1)  1mg 
        
 Recombinant Saccharomyces cerevisiae Thioredoxin-1(TRX1)  1mg 
        
 Recombinant Synechococcus elongatus Thioredoxin-1(trxA)  1mg 
        
 Recombinant Mycobacterium tuberculosis Thioredoxin(trxA)  1mg 
        
 Recombinant Listeria innocua serovar 6a Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus aureus Thioredoxin(trxA)  1mg 
        
 Recombinant Chromatium vinosum Thioredoxin(trxA)  1mg 
        
 Recombinant Mycobacterium bovis Thioredoxin(trxA)  1mg 
        
 Recombinant Listeria monocytogenes serovar 1/2a Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus aureus Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus aureus Thioredoxin(trxA)  1mg 
        
 Recombinant Rhodobacter sphaeroides Thioredoxin(trxA)  1mg 
        
 Recombinant Helicobacter pylori Thioredoxin(trxA)  1mg 
        
 Recombinant Helicobacter pylori Thioredoxin(trxA)  1mg 
        
 Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Thioredoxin(trxA)  1mg 
        
 Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Thioredoxin(trxA)  1mg 
        
 Recombinant Chlamydophila caviae Thioredoxin(trxA)  1mg 
        
 Recombinant Streptomyces coelicolor Thioredoxin(trxA)  1mg 
        
 Recombinant Synechocystis sp. Thioredoxin(trxA)  1mg 
        
 Recombinant Thiobacillus ferrooxidans Thioredoxin(trxA)  1mg 
        
 Recombinant Porphyra yezoensis Thioredoxin(trxA)  1mg 
        
 Recombinant Mycoplasma genitalium Thioredoxin(trxA)  1mg 
        
 Recombinant Porphyra purpurea Thioredoxin(trxA)  1mg 
        
 Recombinant Griffithsia pacifica Thioredoxin(trxA)  1mg 
        
 Recombinant Haemophilus influenzae Thioredoxin(trxA)  1mg 
        
 Recombinant Streptomyces aureofaciens Thioredoxin(trxA)  1mg 
        
 Recombinant Emericella nidulans Thioredoxin(TRX1)  1mg 
        
 Recombinant Neurospora crassa Thioredoxin(trx)  1mg 
        
 Recombinant Cyanidium caldarium Thioredoxin(trxA)  1mg 
        
 Recombinant Penicillium chrysogenum Thioredoxin(TRXA)  1mg 
        
 Recombinant Eubacterium acidaminophilum Thioredoxin(trxA)  1mg 
        
 Recombinant Clostridium litorale Thioredoxin(trxA)  1mg 
        
 Recombinant Bacillus subtilis Thioredoxin(trxA)  1mg 
        
 Recombinant Rhodospirillum rubrum Thioredoxin(trxA)  1mg 
        
 Recombinant Rat Thioredoxin(Txn)  1mg 
        
 Recombinant Chlorobium limicola f.sp. thiosulfatophilum Thioredoxin(trxA)  1mg 
        
 Recombinant Thiocapsa roseopersicina Thioredoxin(trxA)  1mg 
        
 Recombinant Staphylococcus aureus Thioredoxin(trxA)  1mg 
        
 Recombinant Mycoplasma pneumoniae Thioredoxin(trxA)  1mg 
        
 Recombinant Alicyclobacillus acidocaldarius Thioredoxin(trxA)  1mg 
        
 Recombinant Treponema pallidum Thioredoxin(trxA)  1mg 
        
 Recombinant Geodia cydonium Thioredoxin(THIO)  1mg 
        
 Recombinant Chlamydia trachomatis Thioredoxin(trxA)  1mg 
        
 Recombinant Buchnera aphidicola subsp. Schizaphis graminum Thioredoxin(trxA)  1mg 
        
 Recombinant Borrelia burgdorferi Thioredoxin(trxA)  1mg 
        
 Recombinant Mycobacterium smegmatis Thioredoxin(trxA)  1mg 
        
 Recombinant Echinococcus granulosus Thioredoxin(TRX)  1mg 
        
 Recombinant Cyanidioschyzon merolae Thioredoxin(trxA)  1mg 
        
 Recombinant Bovine Thioredoxin(TXN)  1mg 
        
 Recombinant Horse Thioredoxin(TXN)  1mg 
        
 Recombinant Rabbit Thioredoxin(TXN)  1mg 
        
 Recombinant Chicken Thioredoxin(TXN)  1mg 
        
 Recombinant Sheep Thioredoxin(TXN)  1mg 
        
 Recombinant Macaca mulatta (Rhesus macaque) Thioredoxin(TXN)  1mg 
        
 Recombinant Human Thioredoxin(TXN)  1mg 
        
 Recombinant Mouse Thioredoxin(Txn)  1mg 
        
 Recombinant Dog Thioredoxin(TXN)  1mg 
        
 Recombinant Pig Thioredoxin(TXN)  1mg