产品名称: 大鼠柠檬酸合酶(CS)ELISA Kit
英文名称: Rat Citrate synthase,CS ELISA Kit
货号: CSB-E11325r
规格: 96T
种属: Rat
待测物名称: citrate synthase
缩写: CS
蛋白功能1: Biosynthesis/Metabolism
蛋白功能3: Tricarboxylic acid cycle
样本类型: serum, plasma, tissue homogenates, cell lysates
检测范围: 6.25 pg/ml-400 pg/ml
灵敏度: 1.56 pg/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
大鼠柠檬酸合酶(CS)ELISA Kit
用途: For research use only. Not for diagnostic use.
大鼠柠檬酸合酶(CS)ELISA Kit
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CS bound in the initial step. The color development is stopped and the intensity of the color is measured.
大鼠柠檬酸合酶(CS)ELISA Kit
特异性: This assay has high sensitivity and excellent specificity for detection of Rat CS. No significant cross-reactivity or interference between Rat CS and analogues was observed.
大鼠柠檬酸合酶(CS)ELISA Kit
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
大鼠柠檬酸合酶(CS)ELISA Kit
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
大鼠柠檬酸合酶(CS)ELISA Kit
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
大鼠柠檬酸合酶(CS)ELISA Kit
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the CS concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
大鼠柠檬酸合酶(CS)ELISA Kit
产品名称 英文名称 货号 规格
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Pig Citrate synthase, mitochondrial(CS) ELISA kit Pig Citrate synthase, mitochondrial(CS) ELISA kit CSB-EL006031PI 96T
Mouse Citrate synthase, mitochondrial(CS) ELISA kit Mouse Citrate synthase, mitochondrial(CS) ELISA kit CSB-EL006031MO 96T
Chicken Citrate synthase, mitochondrial(CS) ELISA kit Chicken Citrate synthase, mitochondrial(CS) ELISA kit CSB-EL006031CH 96T
Bovine Citrate synthase, mitochondrial(CS) ELISA kit Bovine Citrate synthase, mitochondrial(CS) ELISA kit CSB-EL006031BO 96T
大鼠柠檬酸合酶(CS)ELISA Kit
抗体
产品名称 货号 规格
Rabbit anti-human citrate synthase polyclonal Antibody CSB-PA006031GA01HU 150ul
大鼠柠檬酸合酶(CS)ELISA Kit
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Citrate synthase(gltA) 1mg
Recombinant Synechocystis sp. Citrate synthase(gltA) 1mg
Recombinant Rickettsia slovaca Citrate synthase(gltA) 1mg
Recombinant Citrate synthase(gltA) 1mg
Recombinant Pyrococcus furiosus Citrate synthase(gltA) 1mg
Recombinant Rickettsia bellii Citrate synthase(gltA) 1mg
Recombinant Helicobacter pylori Citrate synthase(gltA) 1mg
Recombinant Deinococcus radiodurans Citrate synthase(gltA) 1mg
Recombinant Dictyostelium discoideum Citrate synthase(gltA) 1mg
Recombinant Citrate synthase(citZ) 1mg
Recombinant Citrate synthase(gltA) 1mg
Recombinant Citrate synthase(gltA) 1mg
Recombinant Citrate synthase(gltA) 1mg
Recombinant Citrate synthase(gltA) 1mg
Recombinant Rickettsia africae Citrate synthase(gltA) 1mg
Recombinant Citrate synthase(gltA) 1mg
Recombinant Citrate synthase(gltA) 1mg
Recombinant Citrate synthase(gltA) 1mg
Recombinant Citrate synthase(gltA) 1mg
Recombinant Citrate synthase(gltA) 1mg
Recombinant Daucus carota Citrate synthase, mitochondrial(CS) 1mg
Recombinant Rhomboid protease AarA(aarA) 1mg
Recombinant Bacillus subtilis Glutamate synthase [NADPH] large chain(gltA) 1mg
Recombinant Circumsporozoite protein(CS) 1mg
Recombinant Escherichia coli Citrate synthase(gltA) 1mg
Recombinant Escherichia coli O6 Citrate synthase(gltA) 1mg
Recombinant Rickettsia prowazekii Citrate synthase(gltA) 1mg
Recombinant Helicobacter pylori Citrate synthase(gltA) 1mg
Recombinant Rickettsia conorii Citrate synthase(gltA) 1mg
Recombinant Rickettsia felis Citrate synthase(gltA) 1mg
Recombinant Bartonella quintana Citrate synthase(gltA) 1mg
Recombinant Bartonella bacilliformis Citrate synthase(gltA) 1mg
Recombinant Rickettsia canadensis Citrate synthase(gltA) 1mg
Recombinant Bartonella vinsonii subsp. berkhofii Citrate synthase(gltA) 1mg
Recombinant Rickettsia typhi Citrate synthase(gltA) 1mg
Recombinant Bartonella henselae Citrate synthase(gltA) 1mg
Recombinant Thiobacillus ferrooxidans Citrate synthase(gltA) 1mg
Recombinant Rickettsia australis Citrate synthase(gltA) 1mg
Recombinant Bartonella vinsonii Citrate synthase(gltA) 1mg
Recombinant Bartonella elizabethae Citrate synthase(gltA) 1mg
Recombinant Corynebacterium glutamicum Citrate synthase(gltA) 1mg
Recombinant Mycobacterium smegmatis Citrate synthase(gltA) 1mg
Recombinant Bacillus coagulans Citrate synthase(ctsA) 1mg
Recombinant Acinetobacter calcoaceticus subsp. anitratus Citrate synthase(gltA) 1mg
Recombinant Thermoplasma acidophilum Citrate synthase(gltA) 1mg
Recombinant Acetobacter aceti Citrate synthase(aarA) 1mg
Recombinant Coxiella burnetii Citrate synthase(gltA) 1mg
Recombinant Streptomyces hygroscopicus Citrate synthase(gltA) 1mg
Recombinant Pseudomonas aeruginosa Citrate synthase(gltA) 1mg
Recombinant Bradyrhizobium japonicum Citrate synthase(gltA) 1mg
Recombinant Populus euphratica Citrate synthase 1mg
Recombinant Sulfolobus solfataricus Citrate synthase(gltA) 1mg
Recombinant Salmonella typhimurium Citrate synthase(gltA) 1mg
Recombinant Antarctic bacterium DS2-3R Citrate synthase(gltA) 1mg
Recombinant Rhizobium meliloti Citrate synthase(gltA) 1mg
Recombinant Human Citrate synthase, mitochondrial(CS) 1mg
Recombinant Pig Citrate synthase, mitochondrial(CS) 1mg
Recombinant Chicken Citrate synthase, mitochondrial(CS) 1mg