重组人蛋白去乙酰1(Human Histone Deacetylase 1/HDAC1)

HDAC1 Human
Histone Deacetylase 1 Human Recombinant
Catalog No. ENZ-424

Synonyms:
HD1, RPD3, GON-10, RPD3L1, DKFZp686H12203, HDAC1, Histone deacetylase 1.

Introduction:
HDAC1 is a member of the histone deacetylase/acuc/apha family and a component of the histone deacetylase complex. HDAC1 is in charge of the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone acetylation and deacetylation, catalyzed by multi-subunit complexes, are a crucial factor in the regulation of eukaryotic gene expression. In addition, HDAC1 interacts with retinoblastoma tumor-suppressor protein and this complex is a major factor in the control of cell proliferation and differentiation. Low levels of HDAC1 were linked to breast cancer progression. Studies have shown that cancer cells successfully maintained decreased levels of pyruvate to prevent inhibition of HDAC1/HDAC3 and thus to escape cell death. HDAC1 is over-expressed in diffuse large B-cell lymphoma and peripheral T-cell lymphoma in opposite to normal lymphoid tissue. The tissue specificity of HDAC1 is ubiquitous, with higher levels in the heart, pancreas and testis, and lower levels in the kidney and brain.

Description:
HDAC1 Human Recombinant fused with 8 amino acid His tag at C-terminus produced in Hi-5 is a single, glycosylated, polypeptide chain containing 490 amino acids (1-482 a.a.) and having a molecular mass of 56.2 kDa.
The HDAC1 is purified by proprietary chromatographic techniques.

Source:
Hi-5 Baculovirus.

Physical Appearance:
Sterile Filtered colorless solution.

Formulation:
The HDAC1 solution contains PBS (pH 7.4) and 10% glycerol.

Stability:
HDAC1 although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
MAQTQGTRRK VCYYYDGDVG NYYYGQGHPM KPHRIRMTHN LLLNYGLYRK MEIYRPHKAN AEEMTKYHSD DYIKFLRSIR PDNMSEYSKQ MQRFNVGEDC PVFDGLFEFC QLSTGGSVAS AVKLNKQQTD IAVNWAGGLH HAKKSEASGF CYVNDIVLAI LELLKYHQRV LYIDIDIHHG DGVEEAFYTT DRVMTVSFHK YGEYFPGTGD LRDIGAGKGK YYAVNYPLRD GIDDESYEAI FKPVMSKVME MFQPSAVVLQ CGSDSLSGDR LGCFNLTIKG HAKCVEFVKS FNLPMLMLGG GGYTIRNVAR CWTYETAVAL DTEIPNELPY NDYFEYFGPD FKLHISPSNM TNQNTNEYLE KIKQRLFENL RMLPHAPGVQ MQAIPEDAIP EESGDEDEDD PDKRISICSS DKRIACEEEF SDSEEEGEGG RKNSSNFKKA KRVKTEDEKE KDPEEKREVT EEEKTKEEKP EAKGVKEEVK LASRHHHHHH.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.