产品名称: 人可溶性胸苷激酶1(TK1)ELISA Kit
英文名称: Human Thymidine Kinase 1,Soluble(TK1)ELISA Kit
货号: CSB-E14261h
规格: 96T
种属: Human
待测物名称: thymidine kinase 1, soluble
缩写: TK1
蛋白功能1: Transcription/Transcription regulation
蛋白功能3: DNA synthesis
样本类型: serum, plasma, tissue homogenates
检测范围: 18.75 pg/ml-1200 pg/ml
灵敏度: 4.69 pg/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
用途: For research use only. Not for diagnostic use.
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
特异性: This assay has high sensitivity and excellent specificity for detection of Human TK1. No significant cross-reactivity or interference between Human TK1 and analogues was observed.
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the TK1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
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蛋白
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Recombinant Thermotoga sp. Thymidine kinase(tdk) CSB-EP023577TNM 1mg E.coli
Recombinant Thermotoga sp. Thymidine kinase(tdk) CSB-YP023577TNM 1mg Yeast
Recombinant Halobacterium salinarum Thymidine kinase(tdk) CSB-EP023577HTL 1mg E.coli
Recombinant Halobacterium salinarum Thymidine kinase(tdk) CSB-YP023577HTL 1mg Yeast
Recombinant Clostridium botulinum Thymidine kinase(tdk) CSB-EP023577DUJ 1mg E.coli
Recombinant Clostridium botulinum Thymidine kinase(tdk) CSB-YP023577DUJ 1mg Yeast
Recombinant Thermoanaerobacter pseudethanolicus Thymidine kinase(tdk) CSB-EP023577TMI 1mg E.coli
Recombinant Thermoanaerobacter pseudethanolicus Thymidine kinase(tdk) CSB-YP023577TMI 1mg Yeast
Recombinant Ureaplasma parvum serovar 3 Thymidine kinase(tdk) CSB-EP023577UBK 1mg E.coli
Recombinant Ureaplasma parvum serovar 3 Thymidine kinase(tdk) CSB-YP023577UBK 1mg Yeast
Recombinant Thermoanaerobacter sp. Thymidine kinase(tdk) CSB-EP023577TMJ 1mg E.coli
Recombinant Thermoanaerobacter sp. Thymidine kinase(tdk) CSB-YP023577TMJ 1mg Yeast
Recombinant Clostridium botulinum Thymidine kinase(tdk) CSB-EP023577DUI 1mg E.coli
Recombinant Clostridium botulinum Thymidine kinase(tdk) CSB-YP023577DUI 1mg Yeast
Recombinant Thymidine kinase(tdk) CSB-EP023577BRL 1mg E.coli
Recombinant Thymidine kinase(tdk) CSB-YP023577BRL 1mg Yeast
Recombinant Oryza sativa subsp. japonica Thymidine kinase(TK) CSB-EP023577OFG 1mg E.coli
Recombinant Oryza sativa subsp. japonica Thymidine kinase(TK) CSB-YP023577OFG 1mg Yeast
Recombinant Encephalitozoon cuniculi Thymidine kinase(TK) CSB-EP023577EKH 1mg E.coli
Recombinant Encephalitozoon cuniculi Thymidine kinase(TK) CSB-YP023577EKH 1mg Yeast
Recombinant Streptomyces coelicolor Thymidine kinase(tdk) CSB-EP023577FOB 1mg E.coli
Recombinant Streptomyces coelicolor Thymidine kinase(tdk) CSB-YP023577FOB 1mg Yeast
Recombinant Vaccinia virus Thymidine kinase(TK) CSB-EP023577VEE 1mg E.coli
Recombinant Vaccinia virus Thymidine kinase(TK) CSB-YP023577VEE 1mg Yeast
Recombinant Geobacillus sp. Thymidine kinase(tdk) CSB-EP023577GFJ 1mg E.coli
Recombinant Geobacillus sp. Thymidine kinase(tdk) CSB-YP023577GFJ 1mg Yeast
Recombinant Deinococcus deserti Thymidine kinase(tdk) CSB-EP023577DIY 1mg E.coli
Recombinant Deinococcus deserti Thymidine kinase(tdk) CSB-YP023577DIY 1mg Yeast
Recombinant Bacillus anthracis Thymidine kinase(tdk) CSB-EP023577BQF 1mg E.coli
Recombinant Bacillus anthracis Thymidine kinase(tdk) CSB-YP023577BQF 1mg Yeast
Recombinant Bacillus anthracis Thymidine kinase(tdk) CSB-EP023577BQG 1mg E.coli
Recombinant Bacillus anthracis Thymidine kinase(tdk) CSB-YP023577BQG 1mg Yeast
Recombinant Clostridium botulinum Thymidine kinase(tdk) CSB-EP023577DUE 1mg E.coli
Recombinant Clostridium botulinum Thymidine kinase(tdk) CSB-YP023577DUE 1mg Yeast
Recombinant Clostridium botulinum Thymidine kinase(tdk) CSB-EP023577DUH 1mg E.coli
Recombinant Clostridium botulinum Thymidine kinase(tdk) CSB-YP023577DUH 1mg Yeast
Recombinant Bacillus cereus Thymidine kinase(tdk) CSB-EP023577BQK 1mg E.coli
Recombinant Bacillus cereus Thymidine kinase(tdk) CSB-YP023577BQK 1mg Yeast
Recombinant Chloroflexus aurantiacus Thymidine kinase(tdk) CSB-EP023577DSW 1mg E.coli
Recombinant Chloroflexus aurantiacus Thymidine kinase(tdk) CSB-YP023577DSW 1mg Yeast
Recombinant Bacillus cereus Thymidine kinase(tdk) CSB-EP023577BQQ 1mg E.coli
Recombinant Bacillus cereus Thymidine kinase(tdk) CSB-YP023577BQQ 1mg Yeast
Recombinant Macrococcus caseolyticus Thymidine kinase(tdk) CSB-EP023577MPA 1mg E.coli
Recombinant Macrococcus caseolyticus Thymidine kinase(tdk) CSB-YP023577MPA 1mg Yeast
Recombinant Dictyoglomus turgidum Thymidine kinase(tdk) CSB-EP023577DKJ 1mg E.coli
Recombinant Dictyoglomus turgidum Thymidine kinase(tdk) CSB-YP023577DKJ 1mg Yeast
Recombinant Chloroflexus aggregans Thymidine kinase(tdk) CSB-EP023577DSV 1mg E.coli
Recombinant Chloroflexus aggregans Thymidine kinase(tdk) CSB-YP023577DSV 1mg Yeast
Recombinant Bacillus cereus Thymidine kinase(tdk) CSB-EP023577BQO 1mg E.coli
Recombinant Bacillus cereus Thymidine kinase(tdk) CSB-YP023577BQO 1mg Yeast
Recombinant Anoxybacillus flavithermus Thymidine kinase(tdk) CSB-EP023577BZO 1mg E.coli
Recombinant Anoxybacillus flavithermus Thymidine kinase(tdk) CSB-YP023577BZO 1mg Yeast
Recombinant Bacillus cereus Thymidine kinase(tdk) CSB-EP023577BQP 1mg E.coli
Recombinant Bacillus cereus Thymidine kinase(tdk) CSB-YP023577BQP 1mg Yeast
Recombinant Bacillus cereus Thymidine kinase(tdk) CSB-EP023577BQL 1mg E.coli
Recombinant Bacillus cereus Thymidine kinase(tdk) CSB-YP023577BQL 1mg Yeast
Recombinant Ureaplasma urealyticum serovar 10 Thymidine kinase(tdk) CSB-EP023577UBL 1mg E.coli
Recombinant Ureaplasma urealyticum serovar 10 Thymidine kinase(tdk) CSB-YP023577UBL 1mg Yeast
Recombinant Dictyoglomus thermophilum Thymidine kinase(tdk) CSB-EP023577DKI 1mg E.coli
Recombinant Dictyoglomus thermophilum Thymidine kinase(tdk) CSB-YP023577DKI 1mg Yeast
人可溶性胸苷激酶1(TK1)ELISA Kit由上海希美化学有限公司为您提供,货号CSB-E14261h,规格:96T,CAS号:CSB-E14261h,如您想了解更多关于人可溶性胸苷激酶1(TK1)ELISA Kit价格、人可溶性胸苷激酶1(TK1)ELISA Kit结构式、批发、用途等信息,欢迎咨询。除供应人可溶性胸苷激酶1(TK1)ELISA Kit外,还可为您提供重组人磷酸激酶1(Human PGK1)、大鼠叠氮胸苷(AZT)ELISA Kit、小鼠叠氮胸苷(AZT)ELISA Kit等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,上海希美化学客户服务电话,售前、售后均可联系。