人磷酸乙醇酸磷酸酶(PGP)ELISA kit

 产品名称： 人磷酸乙醇酸磷酸酶(PGP)ELISA kit
 
 英文名称： Human Phosphoglycolate phosphatase,PGP ELISA kit
 
 货号： CSB-EL017873HU
 
 规格： 96T
 
 种属： Human
 
 待测物名称： phosphoglycolate phosphatase
 
 缩写： PGP
 
 蛋白功能1： Biosynthesis/Metabolism
 
 蛋白功能2： glyconeogenesis and glycometabolism
 
 蛋白功能3： Carbohydrate metabolism
 
 反应时间： 1-5h
 
 所需样本体积： 50-100ul
 
 检测波长： 450 nm
 
 用途： For research use only. Not for diagnostic use.
 
 原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PGP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGP bound in the initial step. The color development is stopped and the intensity of the color is measured. 
 特异性： This assay has high sensitivity and excellent specificity for detection of Human PGP. No significant cross-reactivity or interference between Human PGP and analogues was observed. 
 精密度： Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
 
 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the PGP concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
 
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 Chicken Phosphoglycolate phosphatase(PGP) ELISA kit  Chicken Phosphoglycolate phosphatase(PGP) ELISA kit  CSB-EL017873CH  96T 
        
 Bovine Phosphoglycolate phosphatase(PGP) ELISA kit  Bovine Phosphoglycolate phosphatase(PGP) ELISA kit  CSB-EL017873BO  96T 
        
蛋白 
产品名称 货号 规格 蛋白来源
 Recombinant Probable phosphoglycolate phosphatase(gph)  CSB-EP017873PWC  1mg  E.coli 
        
 Recombinant Probable phosphoglycolate phosphatase(gph)  CSB-YP017873PWC  1mg  Yeast 
        
 Recombinant Pseudomonas putida Probable phosphoglycolate phosphatase(gph)  CSB-EP017873FFZ  1mg  E.coli 
        
 Recombinant Pseudomonas putida Probable phosphoglycolate phosphatase(gph)  CSB-YP017873FFZ  1mg  Yeast 
        
 Recombinant Escherichia coli O157:H7 Phosphoglycolate phosphatase(gph)  CSB-EP017873EOD  1mg  E.coli 
        
 Recombinant Escherichia coli O157:H7 Phosphoglycolate phosphatase(gph)  CSB-YP017873EOD  1mg  Yeast 
        
 Recombinant Escherichia coli Phosphoglycolate phosphatase(gph)  CSB-EP017873ENV  1mg  E.coli 
        
 Recombinant Escherichia coli Phosphoglycolate phosphatase(gph)  CSB-YP017873ENV  1mg  Yeast 
        
 Recombinant Haemophilus influenzae Phosphoglycolate phosphatase(gph)  CSB-EP017873HTA  1mg  E.coli 
        
 Recombinant Haemophilus influenzae Phosphoglycolate phosphatase(gph)  CSB-YP017873HTA  1mg  Yeast 
        
 Recombinant Rhodobacter sphaeroides Phosphoglycolate phosphatase(cbbZ)  CSB-EP017873RLE  1mg  E.coli 
        
 Recombinant Rhodobacter sphaeroides Phosphoglycolate phosphatase(cbbZ)  CSB-YP017873RLE  1mg  Yeast 
        
 Recombinant Nitrosopumilus maritimus Phosphoglycolate phosphatase  CSB-EP017873NFB  1mg  E.coli 
        
 Recombinant Nitrosopumilus maritimus Phosphoglycolate phosphatase  CSB-YP017873NFB  1mg  Yeast 
        
 Recombinant Methanobrevibacter smithii Phosphoglycolate phosphatase  CSB-EP017873MNN  1mg  E.coli 
        
 Recombinant Methanobrevibacter smithii Phosphoglycolate phosphatase  CSB-YP017873MNN  1mg  Yeast 
        
 Recombinant Metallosphaera sedula Phosphoglycolate phosphatase  CSB-EP017873MNM  1mg  E.coli 
        
 Recombinant Metallosphaera sedula Phosphoglycolate phosphatase  CSB-YP017873MNM  1mg  Yeast 
        
 Recombinant Rhodobacter sphaeroides Phosphoglycolate phosphatase(cbbZ)  CSB-EP017873RLG  1mg  E.coli 
        
 Recombinant Rhodobacter sphaeroides Phosphoglycolate phosphatase(cbbZ)  CSB-YP017873RLG  1mg  Yeast 
        
 Recombinant Rhodobacter sphaeroides Phosphoglycolate phosphatase(cbbZ)  CSB-EP017873RIJ  1mg  E.coli 
        
 Recombinant Rhodobacter sphaeroides Phosphoglycolate phosphatase(cbbZ)  CSB-YP017873RIJ  1mg  Yeast 
        
 Recombinant Staphylothermus marinus Phosphoglycolate phosphatase  CSB-EP017873SUP  1mg  E.coli 
        
 Recombinant Staphylothermus marinus Phosphoglycolate phosphatase  CSB-YP017873SUP  1mg  Yeast 
        
 Recombinant Methanoculleus marisnigri Phosphoglycolate phosphatase  CSB-EP017873MNU  1mg  E.coli 
        
 Recombinant Methanoculleus marisnigri Phosphoglycolate phosphatase  CSB-YP017873MNU  1mg  Yeast 
        
 Recombinant Pyrobaculum islandicum Phosphoglycolate phosphatase  CSB-EP017873PZW  1mg  E.coli 
        
 Recombinant Pyrobaculum islandicum Phosphoglycolate phosphatase  CSB-YP017873PZW  1mg  Yeast 
        
 Recombinant Methanosaeta thermophila Phosphoglycolate phosphatase  CSB-EP017873MNW  1mg  E.coli 
        
 Recombinant Methanosaeta thermophila Phosphoglycolate phosphatase  CSB-YP017873MNW  1mg  Yeast 
        
 Recombinant Halobacterium salinarum Phosphoglycolate phosphatase  CSB-EP017873HTL  1mg  E.coli 
        
 Recombinant Halobacterium salinarum Phosphoglycolate phosphatase  CSB-YP017873HTL  1mg  Yeast 
        
 Recombinant Pyrococcus horikoshii Phosphoglycolate phosphatase  CSB-EP017873FHX  1mg  E.coli 
        
 Recombinant Pyrococcus horikoshii Phosphoglycolate phosphatase  CSB-YP017873FHX  1mg  Yeast 
        
 Recombinant Aquifex aeolicus Phosphoglycolate phosphatase(gph)  CSB-EP017873DNV  1mg  E.coli 
        
 Recombinant Aquifex aeolicus Phosphoglycolate phosphatase(gph)  CSB-YP017873DNV  1mg  Yeast 
        
 Recombinant Methanothermobacter thermautotrophicus Phosphoglycolate phosphatase  CSB-EP017873MSR  1mg  E.coli 
        
 Recombinant Methanothermobacter thermautotrophicus Phosphoglycolate phosphatase  CSB-YP017873MSR  1mg  Yeast 
        
 Recombinant Archaeoglobus fulgidus Phosphoglycolate phosphatase  CSB-EP017873DOC  1mg  E.coli 
        
 Recombinant Archaeoglobus fulgidus Phosphoglycolate phosphatase  CSB-YP017873DOC  1mg  Yeast 
        
 Recombinant Rhodobacter capsulatus Phosphoglycolate phosphatase(cbbZ)  CSB-EP017873RLC  1mg  E.coli 
        
 Recombinant Rhodobacter capsulatus Phosphoglycolate phosphatase(cbbZ)  CSB-YP017873RLC  1mg  Yeast 
        
 Recombinant Thermococcus sibiricus Phosphoglycolate phosphatase  CSB-EP017873TMT  1mg  E.coli 
        
 Recombinant Thermococcus sibiricus Phosphoglycolate phosphatase  CSB-YP017873TMT  1mg  Yeast 
        
 Recombinant Halorubrum lacusprofundi Phosphoglycolate phosphatase  CSB-EP017873HUB  1mg  E.coli 
        
 Recombinant Halorubrum lacusprofundi Phosphoglycolate phosphatase  CSB-YP017873HUB  1mg  Yeast 
        
 Recombinant Rhodobacter sphaeroides Phosphoglycolate phosphatase(cbbZ)  CSB-EP017873RLH  1mg  E.coli 
        
 Recombinant Rhodobacter sphaeroides Phosphoglycolate phosphatase(cbbZ)  CSB-YP017873RLH  1mg  Yeast 
        
 Recombinant Thermoproteus neutrophilus Phosphoglycolate phosphatase  CSB-EP017873TNE  1mg  E.coli 
        
 Recombinant Thermoproteus neutrophilus Phosphoglycolate phosphatase  CSB-YP017873TNE  1mg  Yeast 
        
 Recombinant Human Phosphoglycolate phosphatase(PGP)  CSB-EP017873HU  1mg  E.coli 
        
 Recombinant Human Phosphoglycolate phosphatase(PGP)  CSB-YP017873HU  1mg  Yeast