人骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA Kit

 产品名称： 人骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA Kit
 
 英文名称： Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit
 
 货号： CSB-E05128h
 
 规格： 96T
 
 种属： Human
 
 待测物名称： bone gamma-carboxyglutamate (gla) protein
 
 缩写： BGLAP
 
 蛋白功能1： Biosynthesis/Metabolism
 
 蛋白功能3： Biomineralization
 
 样本类型： serum, plasma, tissue homogenates
 
 检测范围： 0.312 ng/ml -20 ng/ml
 
 灵敏度： 0.078 ng/ml
 
 反应时间： 1-5h
 
 所需样本体积： 50-100ul
 
 检测波长： 450 nm
 
 用途： For research use only. Not for diagnostic use.
 
 原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BGLAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BGLAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BGLAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BGLAP bound in the initial step. The color development is stopped and the intensity of the color is measured. 
 特异性： This assay has high sensitivity and excellent specificity for detection of Human BGLAP. No significant cross-reactivity or interference between Human BGLAP and analogues was observed. 
 精密度： Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
 
 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the BGLAP concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
 
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蛋白 
产品名称 货号 规格 蛋白来源
 Recombinant Macaca nemestrina Osteocalcin(BGLAP)  CSB-EP002682MOX  1mg  E.coli 
        
 Recombinant Macaca nemestrina Osteocalcin(BGLAP)  CSB-YP002682MOX  1mg  Yeast 
        
 Recombinant Ateles geoffroyi Osteocalcin(BGLAP)  CSB-EP002682AVF  1mg  E.coli 
        
 Recombinant Ateles geoffroyi Osteocalcin(BGLAP)  CSB-YP002682AVF  1mg  Yeast 
        
 Recombinant Macaca fascicularis Osteocalcin(BGLAP)  CSB-EP002682MOV  1mg  E.coli 
        
 Recombinant Macaca fascicularis Osteocalcin(BGLAP)  CSB-YP002682MOV  1mg  Yeast 
        
 Recombinant Xiphias gladius Osteocalcin(bglap)  CSB-EP002682XAR  1mg  E.coli 
        
 Recombinant Xiphias gladius Osteocalcin(bglap)  CSB-YP002682XAR  1mg  Yeast 
        
 Recombinant Rat Osteocalcin(Bglap)  CSB-EP002682RA  1mg  E.coli 
        
 Recombinant Rat Osteocalcin(Bglap)  CSB-YP002682RA  1mg  Yeast 
        
 Recombinant Lepomis macrochirus Osteocalcin(bglap)  CSB-EP002682LEG  1mg  E.coli 
        
 Recombinant Lepomis macrochirus Osteocalcin(bglap)  CSB-YP002682LEG  1mg  Yeast 
        
 Recombinant Sparus aurata Osteocalcin(bglap)  CSB-EP002682FJX  1mg  E.coli 
        
 Recombinant Sparus aurata Osteocalcin(bglap)  CSB-YP002682FJX  1mg  Yeast 
        
 Recombinant Xenopus laevis Osteocalcin(bglap)  CSB-EP002682XBE  1mg  E.coli 
        
 Recombinant Xenopus laevis Osteocalcin(bglap)  CSB-YP002682XBE  1mg  Yeast 
        
 Recombinant Dromaius novaehollandiae Osteocalcin(BGLAP)  CSB-EP002682DKU  1mg  E.coli 
        
 Recombinant Dromaius novaehollandiae Osteocalcin(BGLAP)  CSB-YP002682DKU  1mg  Yeast 
        
 Recombinant Macropus eugenii Osteocalcin(BGLAP)  CSB-EP002682MPE  1mg  E.coli 
        
 Recombinant Macropus eugenii Osteocalcin(BGLAP)  CSB-YP002682MPE  1mg  Yeast 
        
 Recombinant Camelus bactrianus Osteocalcin(BGLAP)  CSB-EP002682CVZ  1mg  E.coli 
        
 Recombinant Camelus bactrianus Osteocalcin(BGLAP)  CSB-YP002682CVZ  1mg  Yeast 
        
 Recombinant Camelops hesternus Osteocalcin(BGLAP)  CSB-EP002682CBQ  1mg  E.coli 
        
 Recombinant Camelops hesternus Osteocalcin(BGLAP)  CSB-YP002682CBQ  1mg  Yeast 
        
 Recombinant Camelus dromedarius Osteocalcin(BGLAP)  CSB-EP002682CYV  1mg  E.coli 
        
 Recombinant Camelus dromedarius Osteocalcin(BGLAP)  CSB-YP002682CYV  1mg  Yeast 
        
 Recombinant Lama guanicoe Osteocalcin(BGLAP)  CSB-EP002682LBT  1mg  E.coli 
        
 Recombinant Lama guanicoe Osteocalcin(BGLAP)  CSB-YP002682LBT  1mg  Yeast 
        
 Recombinant Gorilla gorilla gorilla Osteocalcin(BGLAP)  CSB-EP002682GGZ  1mg  E.coli 
        
 Recombinant Gorilla gorilla gorilla Osteocalcin(BGLAP)  CSB-YP002682GGZ  1mg  Yeast 
        
 Recombinant Pan troglodytes Osteocalcin(BGLAP)  CSB-EP002682EQV  1mg  E.coli 
        
 Recombinant Pan troglodytes Osteocalcin(BGLAP)  CSB-YP002682EQV  1mg  Yeast 
        
 Recombinant Bison priscus Osteocalcin(BGLAP)  CSB-EP002682BGT  1mg  E.coli 
        
 Recombinant Bison priscus Osteocalcin(BGLAP)  CSB-YP002682BGT  1mg  Yeast 
        
 Recombinant Danio rerio Osteocalcin(bglap)  CSB-EP002682DIL  1mg  E.coli 
        
 Recombinant Danio rerio Osteocalcin(bglap)  CSB-YP002682DIL  1mg  Yeast 
        
 Recombinant Homo sapiens neanderthalensis Osteocalcin(BGLAP)  CSB-EP002682HHR  1mg  E.coli 
        
 Recombinant Homo sapiens neanderthalensis Osteocalcin(BGLAP)  CSB-YP002682HHR  1mg  Yeast 
        
 Recombinant Halobatrachus didactylus Osteocalcin(bglap)  CSB-EP002682HBN  1mg  E.coli 
        
 Recombinant Halobatrachus didactylus Osteocalcin(bglap)  CSB-YP002682HBN  1mg  Yeast 
        
 Recombinant Pongo pygmaeus Osteocalcin(BGLAP)  CSB-EP002682EXP  1mg  E.coli 
        
 Recombinant Pongo pygmaeus Osteocalcin(BGLAP)  CSB-YP002682EXP  1mg  Yeast 
        
 Recombinant Macaca mulatta (Rhesus macaque) Osteocalcin(BGLAP)  CSB-EP002682  1mg  E.coli 
        
 Recombinant Macaca mulatta (Rhesus macaque) Osteocalcin(BGLAP)  CSB-YP002682  1mg  Yeast 
        
 Recombinant Bovine Osteocalcin(BGLAP)  CSB-EP002682BO  1mg  E.coli 
        
 Recombinant Bovine Osteocalcin(BGLAP)  CSB-YP002682BO  1mg  Yeast 
        
 Recombinant Chicken Osteocalcin(BGLAP)  CSB-EP002682CH  1mg  E.coli 
        
 Recombinant Chicken Osteocalcin(BGLAP)  CSB-YP002682CH  1mg  Yeast 
        
 Recombinant Cat Osteocalcin(BGLAP)  CSB-EP002682CA  1mg  E.coli 
        
 Recombinant Cat Osteocalcin(BGLAP)  CSB-YP002682CA  1mg  Yeast 
        
 Recombinant Human Osteocalcin(BGLAP)  CSB-EP002682HU  1mg  E.coli 
        
 Recombinant Human Osteocalcin(BGLAP)  CSB-YP002682HU  1mg  Yeast 
        
 Recombinant Rabbit Osteocalcin(BGLAP)  CSB-EP002682RB  1mg  E.coli 
        
 Recombinant Rabbit Osteocalcin(BGLAP)  CSB-YP002682RB  1mg  Yeast 
        
 Recombinant Goat Osteocalcin(BGLAP)  CSB-EP002682GO  1mg  E.coli 
        
 Recombinant Goat Osteocalcin(BGLAP)  CSB-YP002682GO  1mg  Yeast 
        
 Recombinant Mouse Osteocalcin(Bglap)  CSB-EP002682MO  1mg  E.coli 
        
 Recombinant Mouse Osteocalcin(Bglap)  CSB-YP002682MO  1mg  Yeast 
        
 Recombinant Horse Osteocalcin(BGLAP)  CSB-EP002682HO  1mg  E.coli 
        
 Recombinant Horse Osteocalcin(BGLAP)  CSB-YP002682HO  1mg  Yeast 
        
 Recombinant Dog Osteocalcin(BGLAP)  CSB-EP002682DO  1mg  E.coli 
        
 Recombinant Dog Osteocalcin(BGLAP)  CSB-YP002682DO  1mg  Yeast