人L-氨基酸氧化酶(LAO)ELISA Kit

 产品名称： 人L-氨基酸氧化酶(LAO)ELISA Kit
 
 英文名称： Human L-amino acid oxidase (LAO)ELISA Kit
 
 货号： CSB-E17809h
 
 规格： 96T
 
 种属： Human
 
 待测物名称： interleukin 4 induced 1
 
 缩写： IL4I1
 
 反应时间： 1-5h
 
 所需样本体积： 50-100ul
 
 检测波长： 450 nm
 
 用途： For research use only. Not for diagnostic use.
 
 原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL4I1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL4I1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL4I1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL4I1 bound in the initial step. The color development is stopped and the intensity of the color is measured. 
 特异性： This assay has high sensitivity and excellent specificity for detection of Human IL4I1. No significant cross-reactivity or interference between Human IL4I1 and analogues was observed. 
 精密度： Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
 
 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL4I1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
 
相关产品：
 
产品名称 英文名称 货号 规格
 Mouse L-amino-acid oxidase(IL4I1) ELISA kit  Mouse L-amino-acid oxidase(IL4I1) ELISA kit  CSB-EL011660MO  96T 
        
蛋白 
产品名称 货号 规格 蛋白来源
 Recombinant Sistrurus catenatus edwardsii L-amino-acid oxidase  CSB-EP011660SZP  1mg  E.coli 
        
 Recombinant Sistrurus catenatus edwardsii L-amino-acid oxidase  CSB-YP011660SZP  1mg  Yeast 
        
 Recombinant Crotalus adamanteus L-amino-acid oxidase  CSB-EP011660DYB  1mg  E.coli 
        
 Recombinant Crotalus adamanteus L-amino-acid oxidase  CSB-YP011660DYB  1mg  Yeast 
        
 Recombinant Mus spretus L-amino-acid oxidase(Il4i1)  CSB-EP011660MVA  1mg  E.coli 
        
 Recombinant Mus spretus L-amino-acid oxidase(Il4i1)  CSB-YP011660MVA  1mg  Yeast 
        
 Recombinant Echis ocellatus L-amino-acid oxidase  CSB-EP011660EJL  1mg  E.coli 
        
 Recombinant Echis ocellatus L-amino-acid oxidase  CSB-YP011660EJL  1mg  Yeast 
        
 Recombinant Bothropoides pauloensis L-amino-acid oxidase  CSB-EP011660BUR  1mg  E.coli 
        
 Recombinant Bothropoides pauloensis L-amino-acid oxidase  CSB-YP011660BUR  1mg  Yeast 
        
 Recombinant Naja atra L-amino-acid oxidase  CSB-EP011660NFG  1mg  E.coli 
        
 Recombinant Naja atra L-amino-acid oxidase  CSB-YP011660NFG  1mg  Yeast 
        
 Recombinant Bungarus multicinctus L-amino-acid oxidase  CSB-EP011660BXN  1mg  E.coli 
        
 Recombinant Bungarus multicinctus L-amino-acid oxidase  CSB-YP011660BXN  1mg  Yeast 
        
 Recombinant Bungarus fasciatus L-amino-acid oxidase  CSB-EP011660BXL  1mg  E.coli 
        
 Recombinant Bungarus fasciatus L-amino-acid oxidase  CSB-YP011660BXL  1mg  Yeast 
        
 Recombinant Demansia vestigiata L-amino-acid oxidase  CSB-EP011660DJD  1mg  E.coli 
        
 Recombinant Demansia vestigiata L-amino-acid oxidase  CSB-YP011660DJD  1mg  Yeast 
        
 Recombinant Protobothrops mucrosquamatus L-amino-acid oxidase  CSB-EP011660EZD  1mg  E.coli 
        
 Recombinant Protobothrops mucrosquamatus L-amino-acid oxidase  CSB-YP011660EZD  1mg  Yeast 
        
 Recombinant Crotalus atrox L-amino-acid oxidase  CSB-EP011660DYC  1mg  E.coli 
        
 Recombinant Crotalus atrox L-amino-acid oxidase  CSB-YP011660DYC  1mg  Yeast 
        
 Recombinant Bothrops leucurus L-amino-acid oxidase  CSB-EP011660BIY  1mg  E.coli 
        
 Recombinant Bothrops leucurus L-amino-acid oxidase  CSB-YP011660BIY  1mg  Yeast 
        
 Recombinant Naja oxiana L-amino-acid oxidase  CSB-EP011660NAL  1mg  E.coli 
        
 Recombinant Naja oxiana L-amino-acid oxidase  CSB-YP011660NAL  1mg  Yeast 
        
 Recombinant Vipera ammodytes ammodytes L-amino-acid oxidase  CSB-EP011660VFO  1mg  E.coli 
        
 Recombinant Vipera ammodytes ammodytes L-amino-acid oxidase  CSB-YP011660VFO  1mg  Yeast 
        
 Recombinant Bothrops insularis L-amino-acid oxidase  CSB-EP011660BIX  1mg  E.coli 
        
 Recombinant Bothrops insularis L-amino-acid oxidase  CSB-YP011660BIX  1mg  Yeast 
        
 Recombinant Bothrops jararaca L-amino-acid oxidase  CSB-EP011660BUW  1mg  E.coli 
        
 Recombinant Bothrops jararaca L-amino-acid oxidase  CSB-YP011660BUW  1mg  Yeast 
        
 Recombinant Bothrops alternatus L-amino-acid oxidase  CSB-EP011660BUS  1mg  E.coli 
        
 Recombinant Bothrops alternatus L-amino-acid oxidase  CSB-YP011660BUS  1mg  Yeast 
        
 Recombinant Bothrops marajoensis L-amino-acid oxidase  CSB-EP011660BUY  1mg  E.coli 
        
 Recombinant Bothrops marajoensis L-amino-acid oxidase  CSB-YP011660BUY  1mg  Yeast 
        
 Recombinant Agkistrodon contortrix laticinctus L-amino-acid oxidase  CSB-EP011660AYN  1mg  E.coli 
        
 Recombinant Agkistrodon contortrix laticinctus L-amino-acid oxidase  CSB-YP011660AYN  1mg  Yeast 
        
 Recombinant Bothrops atrox L-amino-acid oxidase  CSB-EP011660BUU  1mg  E.coli 
        
 Recombinant Bothrops atrox L-amino-acid oxidase  CSB-YP011660BUU  1mg  Yeast 
        
 Recombinant Crotalus durissus cascavella L-amino-acid oxidase  CSB-EP011660CST  1mg  E.coli 
        
 Recombinant Crotalus durissus cascavella L-amino-acid oxidase  CSB-YP011660CST  1mg  Yeast 
        
 Recombinant Vipera berus berus L-amino-acid oxidase  CSB-EP011660VDI  1mg  E.coli 
        
 Recombinant Vipera berus berus L-amino-acid oxidase  CSB-YP011660VDI  1mg  Yeast 
        
 Recombinant Bothrops pirajai L-amino-acid oxidase  CSB-EP011660BVB  1mg  E.coli 
        
 Recombinant Bothrops pirajai L-amino-acid oxidase  CSB-YP011660BVB  1mg  Yeast 
        
 Recombinant Eristicophis macmahoni L-amino-acid oxidase  CSB-EP011660EGH  1mg  E.coli 
        
 Recombinant Eristicophis macmahoni L-amino-acid oxidase  CSB-YP011660EGH  1mg  Yeast 
        
 Recombinant Naja kaouthia L-amino-acid oxidase  CSB-EP011660NAF  1mg  E.coli 
        
 Recombinant Naja kaouthia L-amino-acid oxidase  CSB-YP011660NAF  1mg  Yeast 
        
 Recombinant Protobothrops flavoviridis L-amino-acid oxidase  CSB-EP011660EZB  1mg  E.coli 
        
 Recombinant Protobothrops flavoviridis L-amino-acid oxidase  CSB-YP011660EZB  1mg  Yeast 
        
 Recombinant Aplysia dactylomela L-amino-acid oxidase  CSB-EP011660AML  1mg  E.coli 
        
 Recombinant Aplysia dactylomela L-amino-acid oxidase  CSB-YP011660AML  1mg  Yeast 
        
 Recombinant Siganus canaliculatus L-amino-acid oxidase  CSB-EP011660SGJ  1mg  E.coli 
        
 Recombinant Siganus canaliculatus L-amino-acid oxidase  CSB-YP011660SGJ  1mg  Yeast 
        
 Recombinant Ophiophagus hannah L-amino-acid oxidase  CSB-EP011660OEL  1mg  E.coli 
        
 Recombinant Ophiophagus hannah L-amino-acid oxidase  CSB-YP011660OEL  1mg  Yeast 
        
 Recombinant Macrovipera lebetina L-amino-acid oxidase  CSB-EP011660MPJ  1mg  E.coli 
        
 Recombinant Macrovipera lebetina L-amino-acid oxidase  CSB-YP011660MPJ  1mg  Yeast 
        
 Recombinant Calloselasma rhodostoma L-amino-acid oxidase  CSB-EP011660CBG  1mg  E.coli 
        
 Recombinant Calloselasma rhodostoma L-amino-acid oxidase  CSB-YP011660CBG  1mg  Yeast 
        
 Recombinant Mouse L-amino-acid oxidase(Il4i1)  CSB-EP011660MO  1mg  E.coli 
        
 Recombinant Mouse L-amino-acid oxidase(Il4i1)  CSB-YP011660MO  1mg  Yeast