Catalogue Number | ANT-341 | ||
Synonyms | Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367. | ||
Type | Mouse Anti Human Monoclonal. | ||
Introduction | PCNA is located in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps elevate the processivity of leading strand synthesis during DNA replication. In reaction to DNA damage, PCNA is ubiquitinated and takes part in the RAD6-dependent DNA repair pathway. 2 transcript variants encoding the same protein have been found for PCNA gene. Pseudogenes of this PCNA gene have been described on chromosome 4 and on the X chromosome. PCNA is expressed during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA is induced by UV irradiation. | ||
Clone | Pk1A3AT. | ||
Immunogen | Anti-human PCNA mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PCNA amino acids 1-261 purified from E. coli. | ||
Ig Subclass | Mouse IgG3 heavy chain and κ light chain. | ||
Purification Method | PCNA antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. | ||
Protein formulation | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. | ||
Storage Procedures | For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. | ||
Stability / Shelf Life | 12 months at -20°C. 1 month at 4°C. | ||
Applications | PCNA antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000. | ||
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||
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企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
上海希美化学有限公司
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上海市松江区陆家圈路168弄113号
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