Catalogue Number | ANT-347 | ||
Synonyms | HMG1, HMG3, SBP-1, Amphoterin, HMGB1, High-Mobility Group Box 1. | ||
Type | Mouse Anti Human Monoclonal. | ||
Introduction | High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. | ||
Clone | PJ2E1AT. | ||
Immunogen | Anti-human HMGB1 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HMGB1 amino acids 1-215 purified from High Five (Trichoplusia ni) insect cells. | ||
Ig Subclass | Mouse IgG2b heavy chain and κ light chain. | ||
Purification Method | HMGB1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. | ||
Protein formulation | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. | ||
Storage Procedures | For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. | ||
Stability / Shelf Life | 12 months at -20°C. 1 month at 4°C. | ||
Applications | HMGB1 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~300. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:200. | ||
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||
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企业名称
上海希美化学有限公司
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企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
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化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
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上海市松江区陆家圈路168弄113号
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