The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells CD-80 is involved in the costimulatory signal essential for t- lymphocyte activation. t-cell proliferation and cytokine production is induced by the binding of cd28 or ctla-4 to this receptor.
Clone
mB7-1.
Immunogen
Purified mouse LPS-activated B cells.
Ig Subclass
Rat IgG2a.
Purification Method
Ion exchange column.
Protein concentration
1mg/ml in PBS (after reconstitution).
Storage Procedures
Lyophilized: store at 4oC. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.
Reconstitution
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC.
Antibody Properties
Blocking and staining antibody. For staining, use 10 µl/106 cells. Titer for blocking T cell activation should be determined by the investigator.
Available Conjugates
This antibody is only available as purified antibody.
Usage
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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