AKT2 Antibody(S474)

AKT2 Antibody(S474)
Cat.#: T1932
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P31751
Mol Weight: 56kDa
Size: 100ug

Description
AKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.

Recommended Applications
WB, IHC, ELISA

Applications and Recommended Dilution Factors
WB: 1:50~100
IHC: 1:10~50
ELISA: 1:1,000

Species Reactivity
Human

Products Data 

 

Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (S474) (RB11384). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody (S474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specificity
This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S474 of human AKT2.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Alternative Names
AKT2 antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, RAC-beta serine/threonine-protein kinase antibody, RAC-PK-beta antibody

Description References
George, S., et al., Science 304(5675):1325-1328 (2004).
Xu, X., et al., Oncol. Rep. 11(1):25-32 (2004).
Shim, D., et al., Arch. Biochem. Biophys. 425(2):214-220 (2004).
Li, X., et al., Gastroenterology 126(1):122-135 (2004).
Vojtek, A.B., et al., Mol. Cell. Biol. 23(13):4417-4427 (2003).