AF9(MLLT3)Antibody(Center V422)

AF9(MLLT3)Antibody(Center V422)
Cat.#: T1263
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P42568
Mol Weight: 63kDa
Size: 100ug

Description
The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9;11)(p22;q23). The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified; BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Several different structural elements have been identified in AF9, including a colocalizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. In addition, 2 scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border breakpoint regions in 2 leukemic cells lines: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. The patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. A DNA breakage and repair model for nonhomologous recombination between MLL and its partner genes, particularly AF9, has been proposed.

Recommended Applications
WB, ELISA

Applications and Recommended Dilution Factors
WB: 1:100~500
ELISA: 1:1,000

Species Reactivity
Human

Products Data

The anti-MLLT3 Pab is used in Western blot to detect MLLT3 in mouse cerebellum tissue lysate

Specificity
This AF9 (MLLT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MLLT3.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Alternative Names
MLLT3 antibody, AF9 antibody, FLJ2035 antibody, YEATS3 antibody, Protein AF-9 antibody, ALL1-fused gene from chromosome 9 protein antibody, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein antibody, YEATS domain-containing protein 3 antibody

Description References
Iida, S., et al., Oncogene 8(11):3085-3092 (1993).
Nakamura, T., et al., Proc. Natl. Acad. Sci. U.S.A. 90(10):4631-4635 (1993).
Strissel, P. L., et al., Hum. Molec. Genet. 9: 1671-1679 (2000).