Aurora-C(N-term)(AURKC)antibody

Aurora-C(N-term)(AURKC)antibody
Cat.#: 1805-1
Rabbit Monoclonal Antibody
Clone ID: EP1011Y
Swiss Prot: Q9UQB9
Mol Weight: 35kDa
Size: 100ul

Description
Aurora-C is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process (1). Studies show that human Aurora-C is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere protein (INCENP), which are known passenger proteins. INCENP binds and activates Aurora-C in vivo and in vitro. Aurora-C co-expressed with INCENP elicits the phosphorylation of endogenous histone H3 in mammalian cells, even though this phosphorylation is not sufficient to establish chromosome condensation in interphase cells (2). Over-expression of an inactive mutant of Aurora C impaired the localization of Aurora C to the spindle midzone resulting in multinucleation which demonstrates that Aurora C is required for cytokinesis (3).

Recommended Applications
WB

Applications and Recommended Dilution Factors
WB: 1:1000-10,000

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on HepG2 lysate using anti-Aurora C RabMAb (cat. # 1805-1), dilution 1:1000

Specificity
A synthetic peptide corresponding to residues near the N-terminus of human Aurora C was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AURKC antibody, AIE2 antibody, AIK3 antibody, ARK3 antibody, AurC antibody, STK13 antibody, aurora-C antibody, Serine/threonine-protein kinase 13 antibody, Aurora/IPL1/Eg2 protein 2 antibody, Serine/threonine-protein kinase aurora-C antibody

Description References
1. Katayama H, et al. Cancer Metastasis Rev. 22(4): 451-64, 2003
2. Li X, et al. J Biol Chem 279(45): 47201-11, 2004
3. Yan X, et al. Genes Cells 10(6): 617-26, 2005