Aurora-B(AURKB)antibody

Aurora-B(AURKB)antibody
Cat.#: 3315-1
Rabbit Monoclonal Antibody
Clone ID: EPR1010
Swiss Prot: Q96GD4
Mol Weight: 39kDa
Size: 100ul

Description
Aurora-B is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process. Aurora-B is known to be involved in the regulation of centrosome function, bipolar spindle assembly and chromosome segregation. Aurora kinases are localized at the centrosomes of interphase cells, at the poles of the bipolar spindle and in the midbody of the mitotic apparatus. Substrates identified for the Aurora-B kinases include a kinesin-like motor protein, spindle apparatus proteins, histone H3 protein, kinetochore protein and the tumor suppressor protein p53 (1). Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that overexpressed aurora B has multiple functions in cancer development. Identification of Aurora kinases as RasGAP Src homology 3 domain binding protein, also implicates these kinases as potential effectors in the Ras pathway relevant to oncogenesis (2).

Recommended Applications
WB

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000

Species Reactivity
Human, Mouse, Rat

Species cross-reactivity is based on WB analysis

Products Data

 
Western blot analysis on (A) HeLa treated with nocodazole, (B) HeLa, (C) fetal thymus, and (D) A549 cell lysates using anti-Aurora-B RabMAb (cat. #3315-1).

Specificity
A synthetic peptide corresponding to residues in human Aurora-B was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AURKB antibody, AIK2 antibody, AIM-1 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, IPL1 antibody, STK12 antibody, STK5 antibody, aurkb-sv1 antibody, aurkb-sv2 antibody, Serine/threonine-protein kinase 12 antibody, STK-1 antibody, Serine/threonine-protein kinase aurora-B antibody

Description References
1. Katayama H, et al. Cancer Metastasis Rev. 22(4): 451-64, 2003
2. Abdulla A, et al. Cancer Cell International 5(31), 2005