ATGL(PNPLA2)antibody

ATGL(PNPLA2)antibody
Cat.#: 3370-1
Rabbit Monoclonal Antibody
Clone ID: EPR3444(2)
Swiss Prot: Q96AD5
Mol Weight: 54kDa
Size: 100ul

Description
Adipose triglyceride lipase (ATGL) catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes (1). Activity of ATGL is regulated by an activator protein annotated as α/β-fold domain-containing protein 5 (ABHD5), also known as CGI-58 (comparative gene identification 58) (2). Its highest expression is in adipose tissue (1).

Recommended Applications
WB, ICC

Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
ICC: 1:100 - 250

Species Reactivity
Human, Mouse, Rat

Species cross-reactivity is based on WB analysis

Products Data

 
Western blot analysis on (A) 3T3-L1, (B) A431, (C) A673, (D) human heart, (E) human skeletal muscle, and (F) human adipose tissue lysates using anti-ATGL RabMAb (cat. #3370-1).

Specificity
A synthetic peptide corresponding to residues in human ATGL was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
PNPLA2 antibody, 1110001C14Rik antibody, ATGL antibody, DKFZp667M109 antibody, PEDF-R antibody, TTS-2.2 antibody, TTS2 antibody, Patatin-like phospholipase domain-containing protein 2 antibody, Adipose triglyceride lipase antibody, Calcium-independent phospholipase A2 antibody, Desnutrin antibody, IPLA2-zeta antibody, Pigment epithelium-derived factor antibody, TTS2.2 antibody, Transport-secretion protein 2 antibody

Description References
1. The UniProt Consortium. The Universal Protein Resource (UniProt) in 2010. Nucleic Acids Res. 38:D142-D148 (2010).
2. Chandak PG et al. Efficient phagocytosis requires triacylglycerol hydrolysis by adipose triglyceride lipase. J Biol Chem. 2010 Jun 25;285(26):20192-201.