Atg3(ATG3)antibody

Atg3(ATG3)antibody
Cat.#: 3580-1
Rabbit Monoclonal Antibody
Clone ID: EPR4801
Swiss Prot: Q9NT62
Mol Weight: 40kDa
Size: 100ul

Description
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole (1). Atg3 is an E2-like enzyme involved in autophagy and mitochondrial homeostasis. It catalyzes the conjugation of Atg8-like proteins to phosphatidylethanolamine (PE), which is essential to autophagy. Atg3 also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of Atg12 to itself; Atg12 conjugation to Atg3 plays a role in mitochondrial homeostasis but not in autophagy. Atg7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with Atg3 (2).

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:10,000-50,000
IHC: 1:100 - 250
ICC: 1:100 - 250

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

Specificity
A synthetic peptide corresponding to residues near the N-terminus in human Atg3 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATG3 antibody, APG3 antibody, APG3-LIKE antibody, APG3L antibody, DKFZp564M1178 antibody, FLJ22125 antibody, MGC15201 antibody, PC3-96 antibody, Ubiquitin-like-conjugating enzyme ATG3 antibody, Protein PC3-96 antibody

Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.
2. The UniProt Consortium The Universal Protein Resource (UniProt) in 2010 Nucleic Acids Res. 38:D142-D148 (2010).