Asparagine Synthetase(ASNS)antibody

Asparagine Synthetase(ASNS)antibody
Cat.#: 1732-1
Rabbit Monoclonal Antibody
Clone ID: EP282Y
Swiss Prot: P08243
Mol Weight: 64kDa
Size: 100ul

Description
Asparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells, in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines (1). Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia (2).

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:500-2,000
IHC: 1:50
ICC: 1:50-100

Species Reactivity
Human, Rat

For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only

Products Data  

Specificity
A synthetic peptide corresponding to residues near the C-terminus of human asparagine synthetase was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ASNS antibody, TS11 antibody, Asparagine synthetase [glutamine-hydrolyzing] antibody, Cell cycle control protein TS11 antibody, Glutamine-dependent asparagine synthetase antibody

Description References
1. Gantt, J. S., C.-S. Chiang, G. W. Hatfield, and S. M. Arfin. J. Biol. Chem. 255:4808-4813.
2. Ortega, J. A., M. E. Nesbit, Jr., M. H. Donaldson, R. H. Hittle, J. Weiner, M. Karon, and D. Hammond. 1977. Cancer Res. 37:535-540.

Product References
1. Pos Z, Wiener Z, Pocza P, et al, Histamine Suppresses Fibulin-5 and Insulin-like Growth Factor-II Receptor Expression in Melanoma ,Cancer Res 68 (2008) 1997-2005
[Application: IHC]