Ape 1(APEX1)antibody

Ape 1(APEX1)antibody
Cat.#: 2851-1
Rabbit Monoclonal Antibody
Clone ID: EPR4022
Swiss Prot: P27695
Mol Weight: 36kDa
Size: 100ul

Description
Apurinic endonuclease (Ape 1) functions as a major DNA repair enzyme that is essential for embryogenesis and cell viability (1). It is involved in the base excision repair (BER) pathway and acts as a reduction-oxidation signaling factor to maintain transcription factors in an active reduced state (2). Ape 1 plays a critical role as a transcriptional coactivator for different transcription factors (i.e. AP-1, NF-kappaB, p53) in controlling different cellular processes such as apoptosis, proliferation, and differentiation (3). Elevated levels of Ape 1 have been found in cancers such as ovarian, cervical, and prostate (4).

Recommended Applications
WB, IHC

Applications and Recommended Dilution Factors
WB: 1:10,000-50,000
IHC: 1 : 100 - 250

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data  

Specificity
A synthetic peptide corresponding to residues near the N-terminus in human Ape 1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
APEX1 antibody, APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody, REF-1 antibody, Redox factor-1 antibody

Description References
1. Manvilla et al. Biomol NMR Assign. 2009. 2. Fishel et al. DNA Repair (Amst). 7(2): 177-186, 2008. 3. Tell et al. Antioxid Redox Signal. 7(3-4):367-84, 2005. 4. Evans et al. Mutat Res. 461(2):83-108, 2000.