Apaf-1(APAF1)antibody

Apaf-1(APAF1)antibody
Cat.#: 1038-1
Rabbit Monoclonal Antibody
Clone ID: E38
Swiss Prot: O14727
Mol Weight: 130kDa
Size: 100ul

Description
Apaf-1, or apoptotic protease activating factor 1, is a human protein homolog of the C. elagans apoptotic regulatory protein CED-4 that activates caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms an oligomeric complex with caspase-9 in the presence of cytochrome c and dATP, which leads to caspase-9 activation and subsequent activation of caspase-3 (2, 3). Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains, consequently enhancing the catalytic activity of caspase-9 (4), leading to activation of the caspase signaling cascade and apoptosis.

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:500
IHC: 1:50-1:100
ICC: 1:50-1:100

Species Reactivity
Human, Mouse

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

Specificity
A synthetic peptide corresponding to residues near N-terminus of human Apaf-1 was used as immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
APAF1 antibody, APAF-1 antibody, CED4 antibody, DKFZp781B1145 antibody, Apoptotic protease-activating factor 1 antibody, antibody

Description References
1. Zou, H. et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90(3): 405
2. Saleh, A. et al. Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation. J. Biol. Chem. 274 (25): 17941
3. Hu, Y. et al. Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis. EMBO J. 18(13): 3586
4. Shiozaki, E.N. et al. Oligomerization and activation of caspase-9, induced by Apaf-1 CARD. PNAS 99(7): 4197

Product References
1. Kamitsuji Y, Kuroda J, Kimura S, et al, The Bcr-Abl kinase inhibitor INNO-406 induces autophagy and different modes of cell death execution in Bcr-Abl-positive leukemias ,Cell Death Differ 15 (2008) 1712-1722
[Application: WB]