Amphiphysin(AMPH)antibody

Amphiphysin(AMPH)antibody
Cat.#: 2367-1
Rabbit Monoclonal Antibody
Clone ID: EP2061Y
Swiss Prot: P49418
Mol Weight: 128kDa
Size: 100ul

Description
Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).

Recommended Applications
WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:20,000 - 100,000
IHC: 1:100 - 250
ICC: 1:250 - 500
IP: 1:20
FC: 1:160

Species Reactivity
Human, Mouse, Rat

For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only

Products Data 

Specificity
A synthetic peptide corresponding to residues near C-terminus of human Amphiphysin was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AMPH antibody, AMPH1 antibody, Amphiphysin antibody, antibody

Description References
1. David C, et al. FEBS Lett 351(1):73-9, 1994
2. Yamamoto R, et al. Hum Mol Genet 4(2):265-8, 1995
3. Floyd S, et al. Mol Med 4(1):29-39, 1998