Alpha-Actinin 2(ACTN2)antibody

Alpha-Actinin 2(ACTN2)antibody
Cat.#: 2310-1
Rabbit Monoclonal Antibody

Clone ID: EP2529Y
Swiss Prot: P35609
Mol Weight: 100kDa
Size: 100ul

Description
Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibers, while ACTN3 expression is restricted to a subset of type 2 fibers (2).

Recommended Applications
WB, IHC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:200,000-1,000,000
IHC: 1:100-250
IP: 1:40
FC: 1:100

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

Specificity
A synthetic peptide corresponding to residues on the N-terminus of human Alpha-Actinin 2 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ACTN2 antibody, CMD1AA antibody, Alpha-actinin-2 antibody, Alpha-actinin skeletal muscle isoform 2 antibody, F-actin cross-linking protein antibody

Description References
1. Ylanne J, et al. Structure 9(7):597-604, 2001
2. Mills M et al. Hum Mol Genet 10(13): 1335-46, 2001