Alpha-Actinin 1(ACTN1)antibody
Cat.#: 2358-1
Rabbit Monoclonal Antibody
Clone ID: EP2528Y
Swiss Prot: P12814
Mol Weight: 100kDa
Size: 100ul
Description
Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. Alpha-actinin-1 and -4 (ACTN1, ACTN4) are non-muscle isoforms version of ACTN. These isoforms are commonly expressed in filopodia, lamellipodia, stress fibers, sites of adhesion and intercellular contacts (2).
Recommended Applications
WB, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:500 - 1000
ICC: 1:100-250
IP: 1:40
FC: 1:40
Species Reactivity
Human
Cross reactivity for this product was determined by western blot.
Products Data
A. Western blot analysis on (A) NIH 3T3 (B) human skeletal muscle tissue (C) human heart tissue lysates using anti-alpha-actinin 1 RabMAb (cat.# 2358-1), dilution 1:1000.
Specificity
A synthetic peptide corresponding to residues on the C-terminus of human Alpha-Actinin 1 was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Alternative Names
ACTN1 antibody, FLJ40884 antibody, FLJ54432 antibody, Alpha-actinin-1 antibody, Alpha-actinin cytoskeletal isoform antibody, F-actin cross-linking protein antibody, Non-muscle alpha-actinin-1 antibody
Description References
1. Ylanne J, et al. Structure 9(7):597-604, 2001
2. Otey, CA and Carpen O. Cell Motil. Cytoskeleton 58:104-111, (2004).
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企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
上海希美化学有限公司
公司地址
上海市松江区陆家圈路168弄113号
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