Alk/p80(ALK)antibody

Alk/p80(ALK)antibody
Cat.#: 4204-1
Rabbit Monoclonal Antibody
Clone ID: SP8
Swiss Prot: Q9UM73
Mol Weight: 220kDa
Size: 100ul

Description
ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. The 2;5 chromosomal translocation occurs in most anaplastic large-cell non-Hodgkin's lymphomas arising from activated T lymphocytes. This rearrangement was shown to fuse the NPM nucleolar phosphoprotein gene on chromosome 5q35 to a previously unidentified protein tyrosine kinase gene, ALK, on chromosome 2p23 (1). In the predicted hybrid protein, the amino terminus of NPM is linked to the catalytic domain of ALK. Immunoblotting with anti-ALK antibody shows that ALK is highly expressed in the neonatal brain (2). Also expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. Unscheduled expression of the truncated ALK may contribute to malignant transformation in these lymphomas (3).

Recommended Applications
IHC

Applications and Recommended Dilution Factors
IHC: 1:100

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data

 
A. Immunohistochemical staining of paraffin-embedded human anaplastic lymphoma stained with anti-Alk/p80 RabMAb (Cat. #4204-1)

Specificity
Recombinant protein corresponding to a region, which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.

Storage Condition and Buffer
Store at 2-8° C. Tissue culture supernatant with 0.1% sodium azide and 0.05% BSA. Stable for 12 months from the date of receipt.

Alternative Names
ALK antibody, CD246 antibody, NBLST3 antibody, ALK tyrosine kinase receptor antibody, Anaplastic lymphoma kinase antibody

Description References
1. Morris SW, et al. Science 263(5151):1281-4, 1994.
2. Iwahara T, et al. Oncogene 14(4):439-49, 1997.
3. Benharroch D, et al. Blood 91(6): 2076-84, 1998.