Akt1(AKT1)antibody

Akt1(AKT1)antibody
Cat.#: 1085-1
Rabbit Monoclonal Antibody
Clone ID: E45
Swiss Prot: P31749
Mol Weight: 60kDa
Size: 100ul

Description
Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified.

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
IHC: 1:1,000-1:2,500
ICC: 1:1,000
IP: 1:100

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

Specificity
A synthetic peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. The antibody can detect Akt1 and Akt3. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AKT1 antibody, AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, RAC-alpha serine/threonine-protein kinase antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody

Description References
1. Burgering, B.M. and P.J. Coffer. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376: 599
2. Cardone, M.H., et al. Regulation of cell death protease caspase-9 by phosphorylation. Science 282: 1318
3. Hajduch, E., et al. Protein kinase B (PKB/Akt)--a key regulator of glucose transport? FEBS Lett. 492: 199
4. Cross, D.A., et al. Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature 378: 785-789 (1995).