ADFP(PLIN2)antibody
Cat.#: 3293-1
Rabbit Monoclonal Antibody
Clone ID: EPR3713
Swiss Prot: Q99541
Mol Weight: 48kDa
Size: 100ul
Description
ADFP (Adipose differentiation-related protein) is a major protein associated with the lipid droplets in various types of cells, including macrophage-derived foam cells and lipid-laden liver cells (1). The ADFP protein occurs only transiently in adipocytes. It is present at the surface of nascent lipid droplets in early stages of adipocyte differentiation but reduced dramatically after the onset of perilipin expression, whereas ADFP mRNA remains elevated (2). Up-regulation of ADFP may act to sequester fatty acids as triglycerides in discrete lipid droplets that could protect muscle from the detrimental effects of fatty acids on insulin action and glucose tolerance (3).
Recommended Applications
WB, ICC, FC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:100 - 250
FC: 1:100 - 500
Species Reactivity
Human, Mouse, Rat
Species cross-reactivity is based on WB analysis
Products Data
Western blot analysis on (A) fetal liver, (B) HepG2, (C) JAR, and (D) human milk lysates using anti-ADFP RabMAb (cat. #3293-1).
Immunofluorescent staining of HepG2 cells using anti-ADFP RabMAb (cat. #3293-1).
Western blot analysis on (A) mouse brain and (B) rat brain lysates using anti-ADFP RabMAb (cat. #3293-1).
Specificity
A synthetic peptide corresponding to residues in human ADFP was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Alternative Names
PLIN2 antibody, ADFP antibody, ADRP antibody, MGC10598 antibody, Perilipin-2 antibody, Adipophilin antibody, Adipose differentiation-related protein antibody
Description References
1. Masuda Y, et al. J Lipid Res 47(1):87-98, 2006
2. Sztalryd C, et al. J Biol Chem 281(45):34341-8, 2006
3. Phillips SA, et al. Obes Res 13(8):1321-9, 2005